Herpes Simplex Virus Testing

Detection of herpes simplex virus (HSV) by polymerase chain reaction (PCR), has proven to be the most specific, rapid and sensitive means to diagnose anogenital and central nervous system infections. Studies show the range of sensitivity of PCR detection for HSV DNA to be from 75% to 100% with a specificity of 100%.

By utilizing PCR, HSV has been demonstrated in asymptomatic patients on 28% of days tested versus 8.1% by viral isolation. More importantly, asymptomatic shedding was shown on 60% of days where HSV DNA was measured by PCR. This specific detection by PCR has a significant impact on patient counseling because asymptomatic shedding of HSV DNA can occur to a much broader extent than previously thought.