WT1 Wilms Tumor
Test Code
I WT1
Methodology
Immunohistochemistry
CPT Codes
88342 each marker
Turnaround Time
Less than 2 days
Specimen Requirements
- Surgical samples received in 10% neutral buffered formalin
- Formalin fixed paraffin embedded tissue
- Previously prepared pathology materials (slides,smears, blocks or other samples)
Specimen Stability
Indefinitely at room temperature
Causes for Rejection
Inadequate fixation; Improper labeling
Description
WT1 is a suppressor gene located on chromosome 11p13.Wilms’ Tumor Protein (WT1) has been identified in proliferative mesothelial cells, malignant mesothelioma, ovarian cystadenocarcinoma, gonadoblastoma, nephroblastoma and desmoplastic small round cell tumor. Lung adenocarcinomas rarely stain positive with this antibody.
References
- Sugiyama H.(2010). WT1 (Wilms' tumor gene 1): biology and cancer immunotherapy.Jpn J Clin Oncol.40(5):377-87
- Ordonez NG. (2000). Value of thyroid transcription factor-1, E-cadherin, BG8, WT1, and CD44S immunostaining in distinguishing epithelial pleural mesothelioma from pulmonary and nonpulmonary adenocarcinoma. Am J Surg Pathol 24(4):598-606
- Barnoud R et al.(2000). Immunohistochemical expression of WT1 by desmoplastic small round cell tumor: a comparative study with other small round cell tumors. Am J Surg Pathol.24(6):830-6
- Lucas DR et al. (2003). Sarcomatoid mesothelioma and its histological mimics: a comparative immunohistochemical study. Histopathology.42(3):270-9
