Molecular Pathology Laboratory Network

Apoptosis / Cell Cycle Progression by Immunohistochemistry

Associations
I P21WAF: p21 waf, WAF1, inhibitory gene
I P27KIP: p27Kip, kinase inhibitory protein 1
P PERK1/2: pERK1/2, extracellular signal related kinase
I PRB: pRb, phosphorylated retiniblastoma suppressor protein
Methodology
Immunohistochemistry
CPT Codes
88342 each marker
Turnaround Time
3 days
Specimen Requirements
  • Formalin fixed tissue
  • Formalin fixed paraffin embedded tissue (preferred)
Specimen Stability
Indefinitely at room temperature
Storage & Handling
Ship ambient.In extreme hot weather, ship with a cool pack.
Causes for Rejection
Inadequate fixation; Improper labeling
Description
Results are reviewed by a board-certified pathologist to determine if tissues are positive or negative for the marker. These results may be used to assist in determining appropriate patient therapy or treatment. Immunohistochemistry (IHC) combines the principles of histochemistry with the high degree of molecular specificity of the antibody-antigen reaction. The use of an enhanced-polymer detection system provides many benefits including increased sensitivity, fewer procedural steps as compared to conventional techniques, and a reduction in non-specific background staining. MPLN offers a range of markers by IHC including hematologic, tissue specific, infectious agents and proliferation antigens.
References
  1. Press M et al. (2002). Steroids. 67:799-813.
  2. Perttschuk et al. (1996). Cancer. 77:2514-15.
  3. Battifora et al. (1993). Appl Immunohistochem. 1:39-45.

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