Leukoreduced WBC Count
Test Code
FLOW LKRD
Test Synonyms
Absolute white blood cell counts on leucocyte-reduced blood components
Methodology
Flow cytometry
CPT Codes
Not Applicable
Turnaround Time
24 hours
Specimen Requirements
- 200 uL whole blood product (not EDTA)
- 200 uL platelet concentrate
Specimen Stability
- Whole blood product stable at 2-8°C for 48 hours
- Platelet concentrate stable at 18-25°C for 48 hours
Storage & Handling
Causes for Rejection
Specimens submitted at improper temperature; Not received within 48 hours of collection
Reference Range
A leukocyte reduced component is defined as one with < 5x10^6 residual leukocytes per final component. This includes RBC’s, platelets pheresis and pooled platelets. FDA recommends quality control to indicate with 95% confidence that more than 95% of blood units meet these criteria.
References
- Brecher M. (2005). AABB Technical Manual 15th edition.
- Leucocount Kit product insert. (2004). BDIS. San Jose, CA.
- Center for Biologics Evaluation and Research. Recommendations and Licensure Requirements for Leukocyte-Reduced Blood Products. (1996). Rockville, MD: Food and Drug Administration. NCCLS document.
