B-cell Immunoglobulin Heavy Chain Gene Rearrangement
Test Code
P BCELL
Test Synonyms
B-cell gene rearrangement
Associations
B-cell leukemia, Lymphoma
Methodology
Polymerase chain reaction (PCR), Capillary gel electrophoresis
CPT Codes
83891 – Molecular isolation
83894 – Gel electrophoresis
83900 – Amplfication multiplex, first sequence
83901 x8 – Amplification multiplex, each additional sequence
83912 – Interpretation and report
83894 – Gel electrophoresis
83900 – Amplfication multiplex, first sequence
83901 x8 – Amplification multiplex, each additional sequence
83912 – Interpretation and report
Turnaround Time
3 days; 4 days if specimen is paraffin embedded tissue
Specimen Requirements
- 5.0 mL (min. 3.0 mL) whole blood EDTA
- 3.0 mL (min. 1.0 mL) bone marrow EDTA
- 5 mm^3 bone marrow core biopsy, fresh tissue or frozen tissue in MPLN RPMI
- 3.0 mL (min. 2.0 mL) FNA in MPLN RPMI medium
- Formalin fixed paraffin embedded tissue
Specimen Stability
- EDTA whole blood or bone marrow stable at 18-25°C for 72 hours
- Bone marrow biopsy, fresh tissue or FNA in MPLN RPMI at 2-8°C stable for 72 hours
- Frozen tissue frozen at 20°C stable indefinitely
- Paraffin embedded tissue at 18-25°C stable indefinitely
Storage & Handling
- EDTA whole blood, bone marrow, bone marrow biopsy, fresh tissue or FNA, ship in a Styrofoam container with an ice pack. (Do not allow the ice pack to directly contact the sample.)
- Frozen tissue, ship on dry ice.
- Paraffin embedded tissue, ship ambient.
Causes for Rejection
Insufficient volume; Specimen at incorrect temperature; Incorrect anticoagulant or fixative
Reference Range
No rearrangement detected (germline)
Related Content
Flow Cytometry
Description
Evaluation for suspected clonal B-cell lymphoproliferations when morphologic, immunochemical and/or flow cytometry analyses are inconclusive.
References
- Langerak AW et al. (2007). Polymerase chain reaction-based clonality testing in tissue samples with reactive lymphoproliferations: usefulness and pitfalls. Report of the BIOMED-2 Concerted Action BMH4-CT98-3936. Leukemia. 21:222-229.
- Liu H et al. (2007). A practical strategy for the routine use of BIOMED-2 PCR assays for the detection of B- and T-cell clonality in diagnostic haematopathology. Br J Haematol. 138:31-43.
- Van Dongen JJM et al. (2003). Design and standardization of PCR primers and protocols for detection and clonal immunoglobulin and T cell receptor gene recombinations in suspect lymphoproliferations: Report of the BIOMED-2 Concerned Action BMH4-CT98-3936. Leukemia. 17:2257-2317.
- Van Krieken JHJM et al. (2007). Improved reliability of lymphoma diagnosis via PCR-based clonality testing: Report of the BIOMED-2 Concerted Action BHMA-CT98-3936. Leukemia. 21:201-206.
