BCL6 3q27 Rearrangement
Test Code
F BCL6
Test Synonyms
BCL6 gene; 3q27 rearrangement
Associations
B-cell lymphoma, Non-Hodgkin lymphoma (NHL), Diffuse large B cell, Follicular lymphoma
Methodology
Fluorescence in situ Hybridization (FISH)
CPT Codes
88367 x2 - Morphometric analysis, in situ hybridization, automated
88368 x2 - Morphometric analysis, in situ hybridization, manual
88368 x2 - Morphometric analysis, in situ hybridization, manual
Turnaround Time
3 days
Specimen Requirements
- 2.0 mL (min. 1.0 mL) peripheral blood in sodium heparin, EDTA accepted
- 1.0 mL (min. 0.5 mL) bone marrow in sodium heparin, EDTA accepted
- 5 mm^3 fresh tissue in MPLN RPMI media
- 3.0 mL (min. 2.0 mL) FNA in MPLN RPMI media
- 10% neutral buffered formalin fixed paraffin embedded tissue
Specimen Stability
- Peripheral blood and bone marrow stable at 18-25°C for 72 hours
- Fresh tissue or FNA stable at 2-8°C for 72 hours
Storage & Handling
- Whole blood and bone marrow, ship ambient
- Fresh tissue, FNA or paraffin embedded tissue, ship in a Styrofoam container with an ice pack (Do not allow ice pack to directly contact sample)
Causes for Rejection
Clotted specimen; Specimen exposed to extreme temperature; Anticoagulant toxic to cells; Insufficient number of cells; Improper fixative
Reference Range
FISH results indicate whether rearrangement is present or absent. In a normal cell, two fusion (orange/green, or yellow) signals will be observed representing two intact copies of chromosome 3. In a cell that has a translocation with the breakpoint within 3q27, one fusion signal, one orange signal, and one green signal will be found.
Related Content
Flow Cytometry
Description
The B-cell lymphoma 6 (BCL6) gene is localized to 3q27. Rearrangements of 3q27 may be seen in case diffuse large B-cell lymphoma (30-40%) and follicular lymphoma (5-15%). Structural rearrangements include translocations and microdeletions, with about one-half of the translocations involving the immunoglobulin genes at 14q32, 2p12 and 22q13. The remaining translocations involve 3q27 with a wide variety of other chromosome partners.
FISH can detect this rearrangement in either interphase or metaphase cells.
FISH can detect this rearrangement in either interphase or metaphase cells.
References
- Cuneo A and Huret JL. (2000). 3q27 rearrangements in non Hodgkin lymphoma; t(3;Var)(q27;Var) in non-Hodgkin lymphoma. Atlas Genet Cytogenet Oncol Haematol. http://atlasgeneticsoncology.org/Genes/BCL6ID20.html
- Abramson JS et al. (2005). Advances in the biology and therapy of diffuse large B-cell lymphoma: moving toward a molecularly targeted approach. Blood. 106(4):1164.
