Molecular Pathology Laboratory Network

F CMYC

MYC, 8q24 translocation

Lymphoma, Burkitt, Diffuse large B-cell, Solid tumors, Breast and prostate cancer

Fluorescence in situ Hybridization (FISH)

88367 x2 - Morphometric analysis, in situ hybridization, automated
88368 x2 - Morphometric analysis, in situ hybridization, manual

3 days

• 2.0 mL (min. 1.0 mL) peripheral blood in sodium heparin preferred, EDTA accepted
• 1.0 mL (min. 0.5 mL) bone marrow in sodium heparin preferred, EDTA accepted
• 5 mm^3 fresh tissue in MPLN RPMI media
• 3.0 mL (min. 2.0 mL) FNA in MPLN RPMI media
• 10% neutral buffered formalin fixed paraffin embedded tissue

• Peripheral blood and bone marrow stable at 18-25°C for 72 hours
• Fresh tissue or FNA stable at 2-8°C for 72 hours

• Whole blood and bone marrow, ship ambient
• Fresh tissue, FNA or paraffin embedded tissue, ship in a Styrofoam container with an ice pack (Do not allow ice pack to directly contact sample)

Clotted specimen; Specimen exposed to extreme temperature; Anticoagulant toxic to cells; Insufficient number of cells; Improper fixative

A normal nucleus hybridized with the MYC rearrangement probe set produces a two orange/green (yellow) fusion pattern.
The 8q24 rearrangement in t(2;8), t(8;22) or t(8;14) will generate one orange, one green, and one fusion pattern.

Translocations of chromosome 8 are common aberrations in the B-cell non-Hodgkin lymphoma group. The presence of the typical t(8;14) or the rare variants t(8;22) and t(2;8) has been confirmed in all cases of Burkitt lymphoma and in some cases of Burkitt-like lymphoma and diffuse large B-cell lymphoma. FISH can detect this rearrangement in either interphase or metaphase cells.