Molecular Pathology Laboratory Network

IHC Markers

Test Code-----Name (A-Z)
I AKT------------Akt, protein kinase
I AFP------------Alpha fetoprotein
I ALK------------Anaplastic lymphoma kinase -1
I ANDR---------Androgen receptor
I ANXA1---------Annexin A1, hairy cell, B cell lymphoma
I CALDSM------Anti-Caldesmon, smooth muscle
I BCL2----------BCL2 follicular lymphoma, apoptosis inhibiting protein
I BCL6----------BCL6 follicle center B-cell
I CA19 9-- -----CA 19-9 pancreas, liver, ovary, lung tumors
I CA125---------CA 125 epitheliod malignancies ovary, breast
I CALCT--------Calcitonin
I CALRT--------Calretinin, calcium binding protein
I CEA------------Carcinoembryonic antigen (CEA)
I CATHD--------Cathepsin D, breast carcinoma
I CD1a----------CD1a cortical thymocyctes, Langerhans cells
I CD2------------CD2 thymic, T-cells, NK cell
I CD3------------CD3 pan T-cell antigen
I CD4------------CD4 Helper T-cells
I CD5------------CD5 pan T-cells, B-cell subset, thymic carcinoma
I CD7------------CD7 pan T-cells, NK cells and thymocytes
I CD8------------CD8 Suppressor T-cells
I CD10-----------CD10 Common acute lymphoblastic leukemia antigen
I CD15-----------CD15 epithelial, myeloid, Reed-Sternberg cell
I CD20-----------CD20 pan B-cell
I CD23-----------CD23 activated B-cells
I CD30-----------CD30 Ki-1 activated T-cells, B-cells, Reed-Sternberg cell
I CD31-----------CD31 endothelial cells technical only
I CD34-----------CD34 endothelial, stem cells, stromal cells
I CD43-----------CD43 T-cell, myeloid, B-cell subset, histiocytes
I CD45-----------CD45 leuocyte common antigen
I CD56-----------CD56 NK cell
I CD57-----------CD57 neural, neuroendocrine, NK cells
I CD68-----------CD68 macrophages
I CD79a----------CD79a B-cells, plasma cells
I CD99-----------CD99 Ewings sarcoma PNET
I CD138----------CD138 plasma cells, subset epithelial cells
I CDX2-----------CDX2 colorectal carcinoma
I CHROGRAN--Chromogranin A
I CD117---------c-kit, myeloid and mast cell (CD117)
I CLLGIV--------Collagen IV, basement membrane protein
I CYCLIN--------Cyclin D1/PRAD1 mantle cell lymphoma
I CK5/6----------Cytokeratin 5/6, squamous, mesothelial
I BRST 2--------Cytokeratin 5, p63, CK8/18 breast cocktail
I CK5/P63-------Cytokeratin 5 / p63 tumor suppressor gene cocktail
I CK7-------------Cytokeratin 7, 54kD
I CK8-------------Cytokeratin 8; 35BH11
I CAM52---------Cytokeratin 8 (CAM5.2)
I CK8/18---------Cytokeratin 8/18, adenocarcinoma
I CK19-----------Cytokeratin 19
I CK20-----------Cytokeratin 20
I AE1/AE3-------Cytokeratin cocktail, PAN (AE1/AE3)
I CK HMW------Cytokeratin high molecular weight; 34BE12
I CMV------------Cytomegalovirus
I D2 40-----------D2-40, Podoplanin
I DESMIN--------Desmin filament protein
I DOG1-----------DOG1, derived from GIST 1
I ECAD-----------E-cadherin epithelial cell, ductal-type breast carcinoma
I EGFR1----------EGFR, Epidernal growth factor receptor (HER1)
I BER EP--------Epithelial antigen (BER-EP4)
I ERCC1---------ERCC1, Excision repair cross complementing polypeptide
I EMA-------------Epithelial membrane antigen
I ERA-------------Epithelial related antigen (MOC-31)
I ER---------------Estrogen receptor
I FXIIIA------------Factor XIIIa fibrohistiocytic, dendritic interstitial cells
I FASCIN---------Fascin, Hodgkin's lymphoma, Reed Sternberg cells
I GALECT--------Galectin-3, thyroid neoplasm, anaplastic LCL
I GASTRIN-------Gastrin, G-cell antral/pyloric mucosa
I GLYCPA---------Glycophorin-A
I GRANB----------Granzyme B, cytotoxic T-cell subset, NK cell
I GCDFP15-------Gross cystic disease fluid protein 15
I HAIRY------------Hairy cell leukemia (DBA44)
I PYLORI----------Helicobacter pylori
I HEPAR1---------Hepatocyte antigen (HEP Par-1)
I HER2-------------HER2/neu IVD
I HSV---------------Herpes Simplex virus type I and II
I HHV8-------------HHV8 Human Herpesvirus type 8
I HMB 45----------HMB 45, Melanoma associated marker
I GFAP-------------Human glial fibrillary acidic protein
I INHIBIN----------Inhibin, adrenal cortical, sex chord stromal
I KI67---------------Ki67 (MIB-1) cell proliferation marker
I MelanA-----------Melanoma associated marker/Mart 1
I MC----------------Melanoma cocktail (HMB-45, Melan A)
I MLH1-------------Microsatellite instability MLH1
I MSH2-------------Microsatellite instability MSH2
I MSH6-------------Microsatellite instability MSH6
I MSI----------------Microsatellite instability profile MLH1, MSH2, MSH6, PMS2
I MUM1-------------MUM1, Multiple Myeloma Oncogene 1
I MSA---------------Muscle specific actin
I MPO---------------Myeloperoxidase
I MYOGEN--------Myogenin muscle marker, rhabdomyosarcoma
I MYOGLB--------Myoglobin cardiac, skeletal
I NF----------------Neurofilament
I NSE--------------Neuron-specific enolase (NSE)
I P21WAF---------p21 WAF1, inhibitory gene
I P27KIP----------p27Kip, kinase inhibitory protein 1
I P53--------------p53 tumor suppressor gene protein
I P63NU----------p63 nuclear, tumor suppressor gene protein
I P63PC----------p63 plasma cell, tumor suppressor gene protein
I P504S-----------P504S gene product (AMACR) prostatic adenocarcinoma
I PAX5------------PAX-5, B-cell transcription factor
I PERK1/2-------pERK1/2, extracellular signal related kinase
I PDGFR---------Platelet derived growth factor receptor (PDGFR) alpha
I PIN2------------PIN-2 cocktail (P504S, CK HMW,) prostatic intraepithelial neoplasia
I PIN4------------PIN-4 cocktail (P504S,HMW CK, P63)
I PLAP-----------Placental alkaline phosphatase, germ cell tumors, adenocarcinoma
I PMS2----------Post Meiotic Segratation increased 2
I PRB------------pRb, phosphorylated retinoblastoma suppressor protein
I PROEXC------ProEx™ C biomarker
I PR--------------Progesterone receptor
I PSAP-----------Prostatic acid phosphatase
I PSA------------Prostate specific antigen (PSA)
I RCC-----------Renal cell carcinoma
I S100-----------S-100 protein
I SMA------------Smooth muscle actin
I SMM-----------Smooth muscle myosin
I SOMATO-----Somatostatin, D-cells pancreatic islet cells
I SRV------------Survivin
I SYNPTP------Synaptophysin
I TAG72--------TAG 72, adenocarcinoma
I TDT-----------Terminal deoxynucleotidyl transferase, cortical thymocytes
I THYRGLB----Thyroglobulin
I TTF1----------Thyroid Transcription Factor-1
I TYR-----------Tyrosinase
I VZV------------Varicella zoster virus
I VIM------------Vimentin
I VWF-----------Von Willebrand Factor VIII
I WT1-----------WT1, Wilms tumor
I ZAP70--------ZAP 70, CLL/SLL prognosis

Immunohistochemistry

88342 each marker
88360 each oncology marker (I HER2, I KI67,I ER, I PR,I P53)

Less than 2 days

• Surgical samples received in 10% neutral buffered formalin
• Formalin fixed paraffin embedded tissue
• Previously prepared pathology materials (slides,smears, blocks or other samples)

Send reports, history and differential diagnosis

Indefinitely at room temperature

Ship ambient. In extreme hot weather, ship with a cool pack.

Inadequate fixation; Improper labeling

Marker and tissue specific

Results are reviewed by a board-certified pathologist to determine if tissues are positive or negative for the marker. These results may be used to assist in determining appropriate patient therapy or treatment. Immunohistochemistry combines the principles of histochemistry with the high degree of molecular specificity of the antibody-antigen reaction. The use of an enhanced-polymer detection system provides many benefits, including increased sensitivity, fewer procedural steps as compared to conventional techniques, and a reduction in non-specific background staining. MPLN offers a range of markers by Immunohistochemistry including hematologic, tissue specific, infectious agents and proliferation antigens.