Molecular Pathology Laboratory Network

WARF

WARF; CYP2C9 and VKORC1 allele testing

Cytochrome P450 (CYP2C9 or 2C9), Coumadin

Verigene® Warfarin Metabolism Nucleic Acid Test (IVD)

83891 – Isolation or extraction of highly purified nucleic acid, each
83896 x11 – Nucleic acid probe, each
83908 x3 – Amplification, signal, each
83912 – Interpretation and report

2-3 days

1.0 mL EDTA blood

Stable 4°C for up to 10 days

Ship with ice pack (Do not allow ice pack to directly contact sample)

Less than 1.0 mL blood; Improper specimen labeling

CYP2C9*2 allele detected/not detected
CYP2C9*3 allele detected/not detected
VKORC1 1173C>T allele detected/not detected

Intended for the detection and genotyping of the *2 and *3 alleles of the CYP2C9 gene and a single polymorphism (1173C>T) in the VKORC1 gene as an aid in the identification of patients at risk for increased warfarin sensitivity.

1. McClain MR et al. (2008). A rapid ACCE review of CYP2C9 and VKORC1 allele testing to inform warfarin dosing in adults at elevated risk for thrombotic events to avoid serious bleeding. Genet Med. 10:89-98.
2. Schwarz UI. (2008). Genetic determinants of response to warfarin during initial anticoagulation. NEJM. 358:999-1008.