Chromogenic In-Situ Hybdridization (CISH) Probes
Test Synonyms
CISH, ISH markers
Associations
I KAP ISH-----Kappa
I LAM ISH-----Lambda
I EBER ISH----Epstein Barr virus
I LAM ISH-----Lambda
I EBER ISH----Epstein Barr virus
Methodology
Chromogenic in-situ Hybridization (CISH)
CPT Codes
88368 - Morphometric analysis, in situ hybridization each probe
Turnaround Time
Less than 2 days
Specimen Requirements
- Surgical samples received in 10% neutral buffered formalin or B Plus fixative
- Formalin fixed paraffin embedded tissue
- Previously prepared pathology materials (slides, smears, or other samples)
Specimen Stability
Prepared pathology materials, stable indefinitely at 18-25°C
Storage & Handling
Ship samples at ambient temperature
Causes for Rejection
Improper specimen labeling; Inadequate tissue evaluation; Improper sample submission
Description
CISH is an advanced method for detection of gene amplification using a micro-polymer reaction. This technique eliminates the significant background staining associated with non-specific IgG cross reactivity, which can occur with conventional Kappa and Lambda immunohistochemistry (IHC) antibody preparations. CISH is especially useful for hematologic bone marrow samples and where a plasma cell population is thought to be significant such as in hip fractures.
References
- McNicol AM, Farquharson MA. (1997)In situ hybridization and its diagnostic applications in pathology. J Pathol.182(3):250-61.