Lymphocyte Analysis (T-cell subsets plus CD19% CD56%)

FLOW EX

Test Synonym:

T-cell, B-cell and NK-cell enumeration

CPT Code:

86359 - T-cells, total count
86360 – Absolute CD4 and CD8 count, including ratio
86355 - B-cells, total count
86357 - Natural killer (NK) cells, total count

Turnaround Time:

<24 hours

Methodology:

Flow cytometry, multiparameter analysis

Specimen Requirements:

5.0ml (min. 2.0ml) whole blood EDTA

Causes for Rejection:

Specimens stored at incorrect temperature; Non-viable specimens; Specimens in inappropriate anticoagulant; Too few cells; Hemolysis; Specimen clotted

Specimen Stability:

Specimen stable at 18-25°C for 48 hours

Storage and Handling:

Ship ambient (during the summer a cooled ice pack may be used if it is not allowed to contact the sample)

Reference Range:

See report

Indication:

This test is used to evaluate immunologic status using flow cytometry to quantify lymphocyte subsets. Clinicians order this test for primary immunodeficiencies, initial work up for HIV patients and in situations of immune dysfunction. CD4 Extended provides clinicians with a quantitative analysis of the following major lymphocyte subsets; T-cells, B-cells and NK-cells (natural killer). In addition to the reported parameters of CD4, percentages are reported for CD19+ B-cells and CD56+ natural killer cells.

References:

Maurice R. G. O'Gorman and Janet K. A. Nicholson. (2000) Adoption of Single-Platform Technologies for Enumeration of Absolute T-Lymphocyte Subsets in Peripheral Blood. Clin Diagn Lab Immunol. 7(3): 333–335
Orieji C. Illoh. (2004).Current Applications of Flow Cytometry in the Diagnosis of Primary Immunodeficiency Diseases, Archives of Pathology and Laboratory Medicine: 128(1): 23–31