header
 

BCL1 t (11, 14) by Fluorescence in situ Hybridization

F BCL1

Test Synonym:

IGH/CCND1

CPT Code:

88367 x2 – Morphometric analysis

Turnaround Time:

3 days

Methodology:

Fluorescence in situ hybridization (FISH)

Specimen Requirements:

  • 5.0mL (min. 3.0 mL) peripheral blood in sodium heparin preferred, EDTA accepted
  • 3.0mL (min 1.0mL) bone marrow in sodium heparin preferred, EDTA accepted
  • 5mm3 fresh tissue in MPLN RPMI media
  • 3.0mL (min 2.0mL) FNA in MPLN RPMI  media

    • Causes for Rejection:

    Clotted specimen; specimen exposed to extreme temperature; anticoagulant toxic to cells; insufficient number of cells

    Specimen Stability:

  • Peripheral blood and bone marrow - 72 hours at 18-25°C
  • Fresh tissue or FNA - 72 hours at 2°-8°C

    • Storage and Handling:

  • Whole blood and bone marrow ship ambient
  • Fresh tissue or FNA ship in a styrofoam container with an ice pack (do not allow ice pack to directly contact sample)

    • Reference Range:

    Presence or absence of t(11;14)

    Indication:

    Mantle cell lymphoma can be difficult to distinguish from other B-cell malignant lymphomas. The translocation probe is designed to detect the juxtaposition of the IGH locus (14q32) next to the CCND1 (cyclin D1) gene (11q13) in both metaphase and interphase nuclei. This altered location of the IGH locus is thought to cause over expression of cyclin D1 in mantle cell lymphomas. The t(11;14)(q13;q32) can be detected in approximately 95% of mantle cell lymphomas by FISH analysis. In contrast, PCR can detect the t(11;14) in 30-60% of cases, chromosome analysis can detect up to 70%, and Southern blot up to 50% of the translocations. This assay is an excellent alternative to immunoperoxidase detection of cyclin D1 over-expression.

    References:

    1. Li et al. (1999). Detection of translocation t(11;14)(q13;q32) in mantle cell lymphoma by fluorescence in situ hybridization. Am. J. Pathology 154:51449
    2. Kluin et al. (1997). FISH and related techniques in the diagnosis of lymphoma. Cancer Surv 30:3
    3. Gu et al. (2004). Evaluation of peripheral blood involvement of mantle cell lymphoma by fluorescence in situ hybridization in comparison with immunophenotypic and morphologic findings. Mod Pathol 17(5):553.
    4. Sun et al (2003). Fluorescence in situ hybridization: method of choice for a definitive diagnosis of mantle cell lymphoma. Am J Hematol 74(1):78.