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ProEx™C

I PROEXC, I PROEXC TC

Test Synonym:

Immuno, Immunocytochemistry, Immunohistochemistry, Immunoperoxidase, Immunocytology

Test Synonym:

88342 – Immunohistochemistry

Turnaround Time:

3 days

Methodology:

Immunocytochemistry

Specimen Requirements:

  • 1mL ThinPrep® PAP solution
  • 1mL TriPath Imaging SurePath™ Test Pack solution

    • Causes for Rejection:

  • Improper specimen labeling
  • Insufficient sample
  • Inadequate fixation

    • Specimen Stability:

    18°-25°C for 21 days

    Storage and Handling:

    Ship at ambient temperature

    Reference Range:

    Cells positive for aberrant S phase induction not detected

    Indication:

    Results are reviewed by a board-certified pathologist to determine if tissues are positive or negative for proteins present in aberrant S-phase activity. These results may be used to assist in determining appropriate patient therapy or treatment.

    ProExC is a cocktail of monoclonal antibodies that target the Minichromosome Maintenance (MCM) and Topoisomerase II alpha (TOP2A) proteins which, when over-expressed in the cell nucleus, are indicative of disease progression caused by aberrant S-phase induction in the DNA. Human papillomavirus oncoproteins E6 and E7 bypass the critical cell-cycle checkpoints resulting in a prolonged and aberrant S-phase induction cycle. During the transcriptional activation of the aberrant cell cycle, levels of MCM2 and TOP2A proteins increase in proliferating cells.

    References:

    1. Battifora, et al. (1993). Appl  Immunohistochem 1:39-45
    2. Perttschuk, et al. (1996). Cancer 77:2514-15
    3. Press M, et al. (2002). Steroids 67: 799-813