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Leukoreduced WBC Count

LEUKORED


Test Synonym:

Absolute white blood cell counts on leucocyte-reduced blood components

Test Synonym:

Not Applicable

Turnaround Time:

24 hours

Methodology:

Flow cytometry analysis

Specimen Requirements:

  • 200ul whole blood product (not EDTA)
  • 200ul platelet concentrate

    • Causes for Rejection:

    Specimens submitted at improper temperature, or not received within 48 hours of collection

    Specimen Stability:

  • Whole blood product stable at 2-8°C for 48 hours
  • Platelet concentrate stable at 18-25°C for 48 hours

    • Storage and Handling:

    Ship whole blood product with ice pack (do not allow the ice pack to directly contact the sample); ship platelet concentrate ambient

    Reference Range:

    American Association of Blood Banks (AABB) standards stipulate < 5 x 108 leukocytes per transfused unit to prevent febrile transfusion reactions, and < 5 x 106 to prevent cytomegalovirus infection or alloimmunization

    Indication:

    The presence of leucocytes in blood and platelet products is associated with an increased occurrence of febrile transfusion reactions, transmission of cytomegalovirus, and alloimmunization to HLA antigens in transfusion recipients. To minimize these complications, leucoreduction is performed on the blood products. The reduction may be accomplished by apheresis or by a post collection process, using special filters. The optimal level of residual white blood cells is less than 5 x 106 per unit. The FDA has recommended the use of flow cytometry as a counting method for evaluating leucoreduced blood products .

    References:

    1. Leucocount Kit product insert, BDIS, San Jose, CA (06/2004)
    2. Center for Biologics Evaluation and Research. Recommendations and Licensure Requirements for Leukocyte-Reduced Blood Products. Rockville, MD: Food and Drug Administration; 1996. NCCLS document