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UGT1A1 Genotyping

UGT1A1

Test Synonym:

None

Test Synonym:

83891 – Molecular isolation
83892 x8 – Enzymatic digestion
83896 x12 – Nucleic acid probe, each
83903 x4 – Mutation scanning
83908 x4 - Amplification each sequence
83912 – Interpretation and report

Turnaround Time:

2-3 days

Methodology:

Invader™ Third Wave Technologies

Specimen Requirements:

5ml (min. 3ml) EDTA blood preferred, ACD blood accepted.

Causes for Rejection:

  • <3.0ml of whole blood
  • Improper specimen labeling

    • Specimen Stability:

    18°-25° C for 24 hrs; 4° C for up to 4 days

    Storage and Handling:

    Ship at ambient temperature; if shipment delayed, store at 2–8°C and ship with ice pack (do not allow ice pack to directly contact sample)

    Reference Range:

    UGT1A1 *28 allele detected/not detected

    Indication:

    The UGT1A1 blood test is a one-time screening test for newly diagnosed colorectal patients that can provide molecular information about UGT1A1 activity, which is useful for predicting potential risk of irinotecan-induced (Camptosar®) toxicity. This information can then be used as guidance for the determination of appropriate drug dosage prior to initiation of chemotherapy.

    References:

    1. National Cancer Institute: www.cancer.org, www.cancer.gov/cancertopics/types/colon-and-rectal
    2. Third Wave Technologies: www.twt.com/company/pressreleases/2005/aug_22a_2005.html
    3. Food and Drug Administration: www.fda.gov/bbs/topics/NEWS/2005/NEW01220.html
    4. Martinez-Balibrea E et al. (2007). Combined analysis of genetic polymorphisms in thymidylate synthase, uridine diphosphate glucoronosyltransferase and X-ray cross complementing factor 1 genes as a prognostic factor in advanced colorectal cancer patients treated with 5-fluorouracil plus oxaliplatin or irinotecan. Oncol Rep. 17(3):637-45