MPLNET.com » Test Menu » Acute Lymphoblastic Leukemia (ALL) Adult Panel by FISH Client Login | Email | Phone: 1.800.932.2943

Acute Lymphoblastic Leukemia (ALL) Adult Panel by FISH

Test Code

F AALL

Test Synonyms

BCR/ABL t(9;22),TEL/AML1 t(12;21), MLL(KMTA) 11q23

Associations

Acute lymphoblastic leukemia (ALL)

Methodology

Fluorescence in situ Hybridization (FISH)

Turnaround Time

3-5 days

Specimen Requirements

2.0 mL (min. 1.0 mL) peripheral blood in sodium heparin preferred, EDTA accepted
1.0 mL (min. 0.5 mL) bone marrow in sodium heparin preferred, EDTA accepted

Specimen Stability
Peripheral blood and bone marrow stable at 18-25°C for 72 hours
Storage & Handling

Whole blood and bone marrow, ship ambient

Causes for Rejection

Clotted specimen; Specimen exposed to extreme temperature; Anticoagulant toxic to cells; Insufficient number of cells;

Reference Range

See report.

Description

Cytogenetic abnormalities occur in approximately 70% of adult ALL cases. Precursor B-cell acute lymphoblastic leukemia (ALL) occur in 85% of children and 20% of adults with acute leukemia. Abnormalities of chromosome number (hypodiploidy, hyperdiploidy) are more common in ALL than in AML. Hyperdiploidy in children with trisomies of chromosomes 4, 10 and 17 is associated with a good prognosis when structural anomalies are absent. Translocation (12;21) is very difficult to observe with conventional cytogenetic techniques. FISH can detect these abnormalities in interphase and metaphase cells.

References
  1. Graux C, Cools J, Michaux L, et al. (2006). Cytogenetics and molecular genetics of T-cell acute lymphoblastic leukemia: From thymocyte to lymphoblast. Leukemia 20:1496-1510.
  2. Karen Seiter. (2009). Acute Lymphoblastic Leukemia, Medscape.