F AML ETO
AML1/ETO t(8;21)(q22;q22); RUNX1T1
Acute myelogenous leukemia, AML(M2)
Fluorescence in situ Hybridization (FISH)
2.0 mL (min. 1.0 mL) peripheral blood in sodium heparin preferred, EDTA accepted
1.0 mL (min. 0.5 mL) bone marrow in sodium heparin preferred, EDTA accepted
5 mm^3 fresh tissue or 3.0 mL (min. 2.0 mL) FNA in MPLN RPMI media
Whole blood and bone marrow, ship ambient
Fresh tissue and FNA ship in a Styrofoam container with a cool/refrigerated pack (Do not allow cool pack to directly contact sample)
Clotted specimen; Specimen exposed to extreme temperature; Anticoagulant toxic to cells; Insufficient number of cells
FISH results indicate whether rearrangement is present or absent.
In a normal cell, two orange and two green signals will be detected. In a cell with the t(8;21), two fusion signals will be seen representing the two rearranged chromosomes along with one orange and one green signal.
The t(8;21)(q22;q22) is found in 12% of all acute myeloid leukemia (AML) cases, including 40–50% of AML M2 subtype and a small portion of M0, M1 and M4 subtypes of the FAB classification.
FISH can detect this translocation in either interphase or metaphase cells.