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BCL6 3q27 Rearrangement by FISH

Test Code

F BCL6; FP BCL6

Test Synonyms

BCL6 gene; 3q27 rearrangement

Associations

B-cell lymphoma, Non-Hodgkin lymphoma (NHL), Diffuse large B cell, Follicular lymphoma

Methodology

Fluorescence in situ Hybridization (FISH)

Turnaround Time

3-5 days

Specimen Requirements

F BCL6
2.0 mL (min. 1.0 mL) peripheral blood in sodium heparin, EDTA accepted
1.0 mL (min. 0.5 mL) bone marrow in sodium heparin, EDTA accepted
5 mm^3 fresh tissue or 3.0 mL (min. 2.0 mL) FNA in MPLN RPMI media

FP BCL6
10% neutral buffered formalin fixed paraffin embedded tissue (FFPE)

Specimen Stability
Peripheral blood and bone marrow stable at 18-25°C for 72 hours
Fresh tissue or FNA stable at 2-8°C for 72 hours
FFPE stable stable indefinitely
Storage & Handling

Whole blood and bone marrow, ship ambient
Fresh tissue, FNA or FFPE ship in a Styrofoam container with a cool/refrigerated pack (Do not allow cool pack to directly contact sample)

Causes for Rejection

Clotted specimen; Specimen exposed to extreme temperature; Anticoagulant toxic to cells; Insufficient number of cells; Improper fixative

Reference Range

FISH results indicate whether rearrangement is present or absent. In a normal cell, two fusion (orange/green, or yellow) signals will be observed representing two intact copies of chromosome 3. In a cell that has a translocation with the breakpoint within 3q27, one fusion signal, one orange signal, and one green signal will be found.

Description

The B-cell lymphoma 6 (BCL6) gene is localized to 3q27. Rearrangements of 3q27 may be seen in case diffuse large B-cell lymphoma (30-40%) and follicular lymphoma (5-15%).  Structural rearrangements include translocations and microdeletions, with about one-half of the translocations involving the immunoglobulin genes at 14q32, 2p12 and 22q13. The remaining translocations involve 3q27 with a wide variety of other chromosome partners.
FISH can detect this rearrangement in either interphase or metaphase cells.

References
  1. Cuneo A and Huret JL. (2000). 3q27 rearrangements in non Hodgkin lymphoma; t(3;Var)(q27;Var) in non-Hodgkin lymphoma. Atlas Genet Cytogenet Oncol Haematol. http://atlasgeneticsoncology.org/Genes/BCL6ID20.html
  2. Abramson JS et al. (2005).  Advances in the biology and therapy of diffuse large B-cell lymphoma: moving toward a molecularly targeted approach. Blood. 106(4):1164.