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Breast Markers by Immunohistochemistry (IHC)

Test Code

See associations below

Associations
Test Code Description
I BCL2 BCL2, follicular lymphoma, apoptosis inhibiting protein
I BER EP4 Epithelial antigen, BER-EP4
I CD117 CD117, c-kit, myeloid and mast cell
I CK7/8 CAM5.2
I CK HMW Cytokeratin high molecular weight; 34BE12
I CK5/6 Cytokeratin 5/6 usual ductal hyperplasia
I CK7 Cytokeratin 7, 54kD
I CK8/18 Cytokeratin 8/18 aytpical ductal hyperplasia
I CK20 Cytokeratin 20
I ECAD E-cadherin epithelial cell, ductal-type breast
I ER Estrogen receptor
I GCDFP15 Gross cystic disease fluid protein 1
I HER2 HER2/neu (IVD)
I KI67 Ki67 cell proliferation marker
I P53 p53 tumor suppressor gene protein, prognostic
I P63 p63 nucelar, tumor supressor gene protein
I PLAP Placental alkaline phosphatase, germ cell tumors, adenocarcinoma
I PR Progesterone receptor
I SMA Smooth muscle actin
  * Performed at affiliate laboratory
I CATHD* Cathepsin D
I CK 8* Cytokeratin 8
I CK19* Cytokeratin 19

 

Methodology

Immunohistochemistry
Technical component (TC) available for all markers

Turnaround Time

2 days

Specimen Requirements

Paraffin embedded tissue block
3 slides (3-5 uM) per marker on adhesion glass


Please note:
If submitting HER2 protein over-expression by immunohistochemistry,
HER2(ERBB2) gene amplification by in situ hybridization or estrogen /
progesterone receptor expression by immunohistochemistry, the specimen must
follow fixation guidelines listed below:
1. Specimens should be immersed in fixative within one hour of the biopsy or
resection.
2. If delivery of a resection specimen to the pathology department is delayed (eg,
specimens from remote sites), the tumor should be bisected prior to the
immersion in fixative. In such cases, it is important that the surgeon ensure
that the identity of the resection margins is retained in the bisected specimen;
alternatively, the margins may be separately submitted.
3. The time of removal of the tissue and the time of immersion of the tissue in
fixative should be recorded and submitted to the laboratory.

Specimen Stability
Indefinitely at room temperature
Storage & Handling

Ship ambient. Protect from extreme temperature with an ice pack. Separate ice pack from specimen.

Causes for Rejection

Inadequate fixation; Improper labeling

Reference Range

Marker and tissue specific

Description

Results are reviewed by a board-certified pathologist to determine if tissues are positive or negative for the marker. These results may be used to assist in determining appropriate patient therapy or treatment. Immunohistochemistry combines the principles of histochemistry with the high degree of molecular specificity of the antibody-antigen reaction. The use of an enhanced-polymer detection system provides many benefits including increased sensitivity, fewer procedural steps as compared to conventional techniques, and a reduction in non-specific background staining.

MPLN offers a range of markers by Immunohistochemistry including hematologic, tissue specific, infectious agents and proliferation antigens.

References

1. Press M et al. (2002). Steroids. 67:799-813.
2. Perttschuk et al. (1996). Cancer. 77:2514-15.
3. Battifora et al. (1993). Appl Immunohistochem. 1:39-45.