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Breast Markers by Immunohistochemistry (IHC)

Test Code

See associations below

Associations
Test Code Description
I BCL2 BCL2, follicular lymphoma, apoptosis inhibiting protein
I BER EP4 Epithelial antigen, BER-EP4
I CD117 CD117, c-kit, myeloid and mast cell
I CK7/8 CAM5.2
I CK HMW Cytokeratin high molecular weight; 34BE12
I CK5/6 Cytokeratin 5/6 usual ductal hyperplasia
I CK7 Cytokeratin 7, 54kD
I CK8/18 Cytokeratin 8/18 aytpical ductal hyperplasia
I CK20 Cytokeratin 20
I ECAD E-cadherin epithelial cell, ductal-type breast
I ER  Estrogen receptor
I GCDFP15 Gross cystic disease fluid protein 1
I HER2 HER2/neu (IVD)
I KI67 Ki67 cell proliferation marker
I P53 p53 tumor suppressor gene protein, prognostic
I P63NU p63 nucelar, tumor supressor gene protein
I PLAP Placental alkaline phosphatase, germ cell tumors, adenocarcinoma
I PR Progesterone receptor
I SMA Smooth muscle actin
  * Performed at affiliate laboratory
I CATHD* Cathepsin D
I CK 8* Cytokeratin 8
I CK19* Cytokeratin 19

 

Methodology

Immunohistochemistry
Technical component (TC) available for all markers

Turnaround Time

2 days

Specimen Requirements

Formalin fixed tissue
Formalin fixed paraffin embedded tissue (preferred)

Specimen Stability
Indefinitely at room temperature
Storage & Handling

Ship ambient.
In extreme hot weather, ship with a cool pack.

Causes for Rejection

Inadequate fixation; Improper labeling

Reference Range

Marker and tissue specific

Description

Results are reviewed by a board-certified pathologist to determine if tissues are positive or negative for the marker. These results may be used to assist in determining appropriate patient therapy or treatment. Immunohistochemistry combines the principles of histochemistry with the high degree of molecular specificity of the antibody-antigen reaction. The use of an enhanced-polymer detection system provides many benefits including increased sensitivity, fewer procedural steps as compared to conventional techniques, and a reduction in non-specific background staining.

MPLN offers a range of markers by Immunohistochemistry including hematologic, tissue specific, infectious agents and proliferation antigens.

References

1. Press M et al. (2002). Steroids. 67:799-813.
2. Perttschuk et al. (1996). Cancer. 77:2514-15.
3. Battifora et al. (1993). Appl Immunohistochem. 1:39-45.