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Bronchoalveolar Lavage (CD3, CD4, CD8, CD16, CD45, CD4:CD8 ratio)

Test Code

FLOW BAL

Associations

Pulmonary disease, Sarcoidosis, Lung transplantation

Methodology

Flow cytometry, Multiparameter analysis

Turnaround Time

1-2 days

Specimen Requirements

10.0 mL (min. 5 mL) Bronchoalveolar lavage in sterile container with 2-4 mL MPLN RPMI transport media

Specimen Stability
Stable for 48 hours at 4°C
Storage & Handling

Ship with ice pack. Separate ice pack from specimen.

Causes for Rejection

Specimens stored at incorrect temperature; Non-viable specimens; Specimens in inappropriate anticoagulant; Too few cells; Hemolysis; Specimen clotted

Reference Range

See report.

References
  1. Papiris SA et al. (2007). CD8+ T lymphocytes in bronchoalveolar lavage in idiopathic pulmonary fibrosis. Journal of Inflammation.
  2. Reichenberger F et al. (2007). Bronchoalveolar lavage quality influences the T4/T8 ratio in sarcoidosis.  Respiratory Medicine.
  3. Smith PA et al. (2006). Cytometric analysis of BAL T-cells labeled with a standardized antibody cocktail correlates with immunohistochemical staining. Cytometry.
  4. Tutor-Ureta P et al. (2006). Prognostic value of neutrophils and NK cells in bronchoalveolar lavage of sarcoidosis. Cytometry.
  5. Papiris SA et al. (2005). Relationship of BAL and lung tissue CD4+ and CD8+ T lymphocytes, and their ratio in idiopathic pulmonary fibrosis. Chest.