F CMYC, FP CMYC
MYC, 8q24 translocation
Lymphoma, Burkitt, Diffuse large B-cell, Solid tumors, Breast and prostate cancer
Fluorescence in situ Hybridization (FISH)
3 -5 days
2.0 mL (min. 1.0 mL) peripheral blood in sodium heparin preferred, EDTA accepted
1.0 mL (min. 0.5 mL) bone marrow in sodium heparin preferred, EDTA accepted
5 mm^3 fresh tissue or 3.0 mL (min. 2.0 mL) FNA in MPLN RPMI media
10% neutral buffered formalin fixed paraffin embedded tissue
Whole blood and bone marrow, ship ambient
Fresh tissue, FNA or paraffin embedded tissue, ship in a Styrofoam container with a cool/refrigerated pack (Do not allow cool pack to directly contact sample)
Clotted specimen; Specimen exposed to extreme temperature; Anticoagulant toxic to cells; Insufficient number of cells;
A normal nucleus hybridized with the MYC rearrangement probe set produces a two orange/green (yellow) fusion pattern.
The 8q24 rearrangement in t(2;8), t(8;22) or t(8;14) will generate one orange, one green, and one fusion pattern.
Translocations of chromosome 8 are common aberrations in the B-cell non-Hodgkin lymphoma group. The presence of the typical t(8;14) or the rare variants t(8;22) and t(2;8) has been confirmed in all cases of Burkitt lymphoma and in some cases of Burkitt-like lymphoma and diffuse large B-cell lymphoma. FISH can detect this rearrangement in either interphase or metaphase cells.