-7/7q-, -7/del(7)(q31), deletion 7q31
Acute myelogenous leukemia (AML), Myelodysplastic syndrome (MDS)
Fluorescence in situ Hybridization (FISH)
5.0 mL (min. 3.0 mL) peripheral blood in sodium heparin preferred, EDTA accepted
3.0 mL (min 1.0 mL) bone marrow in sodium heparin preferred, EDTA accepted
5 mm^3 fresh tissue or 3.0 mL (min. 2.0 mL) FNA in MPLN RPMI media
Whole blood and bone marrow, ship ambient.
Fresh tissue or FNA, ship in a Styrofoam container with a cool/refrigerated pack. (Do not allow cool pack to directly contact sample)
Clotted specimen; Apecimen exposed to extreme temperature; Anticoagulant toxic to cells; Insufficient number of cells; Improper fixative
In a normal cell, the expected pattern is the two orange, two green signal pattern. In a hybridized abnormal cell containing the deletion, the one orange, two green signal pattern is observed. In an abnormal cell containing a monosomy 7, one orange and one green signal is observed.
Complete or partial loss of chromosome 7, predominantly deletion of 7q, is a recurrent chromosomal aberration found in myeloid disorders such as myelodysplastic syndrome (MDS) or acute myeloid leukemia (AML). Deletion of the long arm of chromosome 7 has been reported in a few cases of chronic lymphoproliferative disorders: splenic marginal zone lymphoma, hairy cell leukemia (HCL) and lymphoplasmacytoid lymphoma (LPL).
Abnormality of chromosome 7 can occur as the sole chromosome change or associated with other cytogenetic defects. Other chromosome changes commonly occurring with 7q- are monosomy 5/5q-, trisomy 8 and 20q-.
FISH can detect this abnormality in either interphase or metaphase cells.