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Chromogenic In-Situ Hybridization (CISH) Probes

Test Code

See associations below

Test Synonyms

CISH, ISH

Associations
Test Code Description
I KAP ISH Kappa
I LAM ISH Lambda
IEBER ISH Epstein Barr virus
  * Sent to referral lab
I HPV P* HPV DNA In-Situ Hybridization
Methodology

Chromogenic in-situ Hybridization (CISH)

Turnaround Time

2 days

Specimen Requirements
  • Surgical samples received in 10% neutral buffered formalin or B Plus fixative
  • Formalin fixed paraffin embedded tissue
  • Previously prepared pathology materials (slides, smears, or other samples)
Specimen Stability
Prepared pathology materials, stable indefinitely at 18-25°C
Storage & Handling

Ship samples at ambient temperature

Causes for Rejection

Improper specimen labeling; Inadequate tissue evaluation; Improper sample submission

Description

CISH is an advanced method for detection of gene amplification using a micro-polymer reaction. This technique eliminates the significant background staining associated with non-specific IgG cross reactivity, which can occur with conventional Kappa and Lambda immunohistochemistry (IHC) antibody preparations.

CISH is especially useful for hematologic bone marrow samples and where a plasma cell population is thought to be significant such as in hip fractures.

References
  1. McNicol AM, Farquharson MA. (1997)In situ hybridization and its diagnostic applications in pathology. J Pathol.182(3):250-61.