Karyotype analysis, cancer cytogenetics
24, 48 and/or 72 hour culture / G-banding
3.0 mL (min. 1.0 mL) bone marrow in sodium heparin5 mm^3 bone marrow core biopsy in MPLN media
Ship with a cool pack (refrigerated).
Call before sending specimen on Friday
Ship Saturday for Monday delivery, and protect specimen from extreme temperature
Send WBC count with requisition when ordering bone marrow cytogenetics
Clotted specimen; Specimen exposed to extreme temperature; Anticoagulant toxic to cells; Insufficient number of cells
Chromosome count and morphology consistent with normal karyotype
Bone marrow is the tissue of choice for cytogenetic study of most hematologic conditions because it more accurately reflects what occurs in vivo than other sample types. In hematological malignant diseases, the recognized recurring chromosomal abnormalities often correlate with particular subtypes of leukemia that have characteristic morphological and clinical features, such as response to therapy.
The study of chromosomal abnormalities in leukemia serves two functions. The first of these is to assist in more accurate diagnosis, thereby providing prognostic information and allowing the more rational sections of therapy for a particular patient; and the second is to identify the sites of consistent rearrangements, providing the precise localization required for the isolation and cloning of DNA from these regions.