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Epstein Barr Virus by ISH

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Test Synonyms



Infectious mononucleosis, Burkitt's lymphoma,Reed Sternberg cells Hodgkin's lymphoma, nasopharyngeal carcinoma, HIV infection


Chromogenic in-situ Hybridization (CISH)

Turnaround Time

2 days

Specimen Requirements
  • Surgical samples received in 10% neutral buffered formalin or B Plus fixative
  • Formalin fixed paraffin embedded tissue
  • Previously prepared pathology materials (slides, smears, or other samples) Send reports, history and differential diagnosis.
    Specimen Stability
    Prepared pathology materials, stable indefinitely at 18-25°C
    Storage & Handling

    Ship samples at ambient temperature

    Causes for Rejection

    Improper specimen labeling; Inadequate tissue evaluation; Improper sample submission

    Reference Range

    EBER by ISH is performed on formalin-fixed paraffin-embedded tissues and the result is very similar to the traditional IHC slide -  a brown (DAB) chromogen target location on a Hematoxylin counter-stained slide.


    EBV infection is associated with a variety of pathological conditions. The virus has been reported to be demonstrated in infectious mononucleosis, Burkitt’s lymphoma, the Reed Sternberg cells of Hodgkin’s disease and in nasopharyngeal carcinoma. In HIV infection, EBV has also been reported to be demonstrated in primary CNS lymphomas and oral hairy leukoplakia lesions. 

    The technique demonstrates cells latently-infected with EBV as it hybridizes to abundantly expressed Epstein-Barr virus-encoded RNA (EBER) transcripts which are concentrated in the nuclei of latently-infected cells.


    McNicol AM, Farquharson MA. (1997)In situ hybridization and its diagnostic applications in pathology. J Pathol.182(3):250-61.