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Flow Cytometry Leukemia / Myeloma / Lymphoma

Test Code

FLOW

Test Synonyms

Immunophenotyping, myeloid / lymphoid markers

Associations

Acute myeloid leukemia, Acute lymphoblastic leukemia, Chronic lymphocytic leukemia, Non-Hodgkin lymphoma

Methodology

Flow cytometry, multiparameter analysis

Turnaround Time

Less than 24 hours

Specimen Requirements
    • 5.0 mL (min. 2.0 mL) whole blood heparin preferred, CytoChex and EDTA or ACD whole blood accepted
    • <2.0 ml (min. 0.5 mL) bone marrow aspirate heparin preferred, EDTA bone marrow accepted
    • 20 mm^3 bone marrow core biopsy in MPLN RPMI transport media
    • 5 mm^3 fresh tissue in MPLN RPMI transport media
    • FNA (cell suspension or core biopsy) in MPLN RPMI transport media (2.0-4.0 mL RPMI)
    • 4.0 mL (min. 2.0 mL) cerebrospinal fluid (CSF) in sterile container
    • 10.0 mL (min. 5.0 mL) pleural/body fluid in sterile container
    • 10.0 mL (min. 5.0 mL) bronchoalveolar lavage in sterile container
    If RPMI media or MPLN RPMI transport media is not available, send in sterile saline.
    Specimen Stability
    Whole blood, bone marrow biopsy, fresh tissue, CSF, pleural fluid, or FNA in MPLN RPMI stable for 48 hours at 2-8°C
    Storage & Handling

    Whole blood, bone marrow, biopsy, fresh tissue, CSF, pleural/body fluid or FNA, ship in a Styrofoam container with a cool pack. (Do not allow cool/refrigerated pack to directly contact samples)

    Causes for Rejection

    Specimens stored at incorrect temperature; Non-viable specimens; Specimens in inappropriate anticoagulant; Too few cells; Hemolysis; Specimen clotted

    Reference Range

    See report

    References
    1. Craig and Foon. (2008). Flow cytometric immunophenotyping for hematologic neoplasms. Blood 111, No. 8:3941-3967.
    2. Special Issue. (2006). Zap-70 and CLL. Cytometry, 70B:4.
    3. Bethesda International Consensus Conference on Flow Cytometric Immunophenotyping of Hematopoietic Neoplasia. (2006).
    4. Davis BH et al. (2006). Bethesda International Consensus Recommendations on the Flow Cytometric Immunophenotypic Analysis of Hematolymphoid Neoplasia: Medical Indications (submitted for publication, Cytometry)
    5. Crespo et al. (2003). ZAP-70 expression as a surrogate for Immunoglobulin-Variable-Region mutations in chronic lymphocytic leukemia. NEJM. 348(18):1764.
    6. Nguyen et al. (2003). Flow Cytometry in Hematopathology. Humana Press.
    7. Shapiro H (2003). Practical Flow Cytometry 4th edition. Wiley-Liss.
    8. Wells et al. (2003). Myeloid and monocytic dyspoiesis as determined by flow cytometric scoring in myelodysplastic syndrome correlates with IPSS and with outcome after hematopoietic stem cell transplantation. Blood 102(1):394-403.
    9. Foucar. (2001). Bone Marrow Pathology 2nd edition. ASCP Press.

     

    Keywords
    flow leukemia immunophenotyping myeloid lymphoid non-hodgkin lymphoma cytometry