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Glioma Panel 1p-, 9q-; EGFR amplification by FISH

Test Code

FP GLIO MAP

Test Synonyms

[1p36-, 19q13- ; EGFR 7p11.2 amp.]

Associations

Oligodendroglioma

Methodology

Fluorescence in situ Hybridization (FISH)

Turnaround Time

3-5 days

Specimen Requirements

10% neutral buffered formalin fixed paraffin embedded tissue
5 mm^3 fresh tissue or 3.0 mL (min. 2.0 mL) FNA in MPLN RPMI media

Specimen Stability
Paraffin blocks stable at 18-25°C indefinitely Fresh tissue or FNA stable at 2-8°C for 72 hours
Storage & Handling

Fresh tissue, FNA or paraffin embedded tissue, ship in a Styrofoam container with a cool/refrigerated pack (Do not allow cool pack to directly contact sample)

Causes for Rejection

Improper fixative; Tissue not fixed; Insufficient tissue; Specimen exposed to extreme temperature

Reference Range

See report

Description

Deletions of chromosome 1p and 19q arms are frequent genetic abnormalities in primary human gliomas and are especially common in oligodendrogliomas. While 1p deletions are relatively common in other types of tumors, 19q deletions appear to be common only in gliomas. EGFR gene amplification and overexpression are a striking feature of glioblastoma (GBM) but are rare in low-grade gliomas,

References
  1. Law, Mark et al. (2006). Cancer Genetics and Cytogenetics. 160:1–14.
  2. Barbashina V et al. (2005). Allelic loss at 1p36 and 19q13 in gliomas: correlation with histologic classification, definition of a 150-kb minimal deleted region at 1p36, and evaluation of CAMTA1 as a candidate tumor suppressor gene. Clin Cancer Research. 11:1119.
  3. Smith JS et al. (2000). Alterations in chromosome arms 1p and 19q as predictors of survival in oligodendrogliomas, astrocytomas, and mixed oligoastrocytomas. J Clin Oncol. 18(3):636.