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HHV8 Human Herpesvirus Type 8 by IHC

Test Code

I HHV8

Associations

Kaposi's sarcoma

Methodology

Immunohistochemistry

Turnaround Time

2 days

Specimen Requirements

Surgical samples received in 10% neutral buffered formalin
Formalin fixed paraffin embedded tissue
Previously prepared pathology materials (slides,smears, blocks or other samples)

Send reports, history and differential diagnosis.

Specimen Stability
Indefinitely at room temperature
Storage & Handling
Causes for Rejection

Inadequate fixation; Improper labeling

Description

Human herpesvirus type 8 (HHV-8) is the likely etiological agent of Kaposi’s sarcoma (KS). HHV-8 DNA sequences have been found in Kaposi’s sarcoma lesions, primary effusion lymphoma, and multicentric Castleman’s disease via polymerase chain reaction and in situ hybridization. Latent nuclear antigen (LNA-1, LNA, LANA-1), also known as ORF73, is a 222- or 234 kD protein that is consistently expressed in HHV-8 infected cells. Anti-HHV-8 labels the latent nuclear antigen protein via immunohistochemistry.

References
  1. Hong A et al. (2003).Immunohistochemical detection of the human herpes virus 8 (HHV8) latent nuclear antigen-1 in Kaposi’s sarcoma.Pathology. 35(5):448-50