IDH mutations are recurrent in a variety of cancer settings including glial neoplasms, acute myeloid leukemias, cholangiocarcinomas, and chondrosarcomas, among others.
Next Generation Sequencing Targeted HotSpots
QIAGEN® Human Tumor Actionable Mutations Panel (GeneRead™ DNAseq Targeted Panels V2) for targeted enrichment
Formalin Fixed Paraffin Embedded tissue block or slides (3-5 slides at 5 micron minimum) NOTE: Include a surgical pathology report with the sample.
5.0 mL (min. 3.0 mL) whole blood EDTA, Sodium Heparin or ACD
3.0 mL (min. 1.0 mL) bone marrow EDTA, Sodium Heparin or ACD
Ship at ambient temperature, cool in summer. (Do not allow cool/refrigerated pack to directly contact sample)
Improper specimen labeling; Insufficient sample volume; Clotted specimen; Specimen older than 7 days
Targeted next generation sequencing is performed to asses for the following pathogenic hotspot mutations: IDH1 p.R132 and IDH2 p.R140 and IDH2p.R172.
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