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IGH 14q32 Rearrangement by FISH

Test Code

F IGH; FP IGH

Test Synonyms

immunoglobulin heavy chain, 14q32 rearrangement

Associations

Non-Hodgkin lymphoma, Myeloma

Methodology

Fluorescence in situ Hybridization (FISH)

Turnaround Time

3-5 days

Specimen Requirements

F IGH
2.0 mL (min. 1.0 mL) peripheral blood in sodium heparin preferred, EDTA accepted
1.0 mL (min. 0.5 mL) bone marrow in sodium heparin preferred, EDTA accepted
5 mm^3 fresh tissue or 3.0 mL (min. 2.0 mL) FNA in MPLN RPMI media

FP IGH
10% neutral buffered formalin fixed paraffin embedded tissue

Specimen Stability
Peripheral blood and bone marrow stable at 18-25°C for 72 hours
Fresh tissue or FNA stable at 2-8°C for 72 hours
FFPE stable indefinitely
Storage & Handling

Whole blood and bone marrow, ship ambient
Fresh tissue, FNA or paraffin embedded tissue, ship in a Styrofoam container with a cool/refrigerated pack (Do not allow cool pack to directly contact sample)

Causes for Rejection

Clotted specimen; Specimen exposed to extreme temperature; Anticoagulant toxic to cells; Insufficient number of cells; Improper fixative

Reference Range

See report

Description

Rearrangements involving the IGH gene have been identified in about 50% of non-Hodgkin B-cell lymphomas (NHLs) and correlated to clinically relevant subgroups. A t(14;18)(q32;q21) is found in 88.1% of follicular lymphomas and a t(11;14)(q13;q32) is in 85.7% mantle cell lymphomas. IGH rearrangements are identified in 52.5% of diffuse large B-cell lymphomas. Rearrangements of the IgH gene occur in 34.7% of plasma cell myeloma cases and is among the most frequent chromosomal change. FISH can detect this rearrangement in either interphase or metaphase cells.

References
  1. Bernicot I et al. (2007). Cytogenet Genome Res. 118:345-352.
  2. Letter to the editor. (2007). Cancer Genetics and Cytogenetics. 172:172-173.
  3. Peinkowska-Grela et al. (2005). Frequent aberrations of chromosome 8 in aggressive B-cell non-Hodgkin lymphoma. Cancer Genet Cytogenet. 156(2):114.