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IGH/MYC t(8;14) by FISH

Test Code

F IGH MYC; FP IGH MYC

Test Synonyms

t(8;14)(q32;q24), MYC

Associations

Burkitt lymphoma

Methodology

Fluorescence in situ Hybridization (FISH)

Turnaround Time

3-5 days

Specimen Requirements

F IGH MYC
2.0 mL (min. 1.0 mL) peripheral blood in sodium heparin, EDTA accepted
1.0 mL (min. 0.5 mL) bone marrow in sodium heparin, EDTA accepted
5 mm^3 fresh tissue or 3.0 mL (min. 2.0 mL) FNA in MPLN RPMI media

FP IGH MYC
10% neutral buffered formalin fixed paraffin embedded tissue

Specimen Stability
Peripheral blood and bone marrow stable at 18-25°C for 72 hours
Fresh tissue or FNA stable at 2-8°C for 72 hours
FFPE stable stable indefinitely
Storage & Handling

Whole blood and bone marrow, ship ambient
Fresh tissue, FNA or paraffin embedded tissue, ship in a Styrofoam container with a cool/refrigerated pack (Do not allow cool pack to directly contact sample)

Causes for Rejection

Clotted specimen; Specimen exposed to extreme temperature; Anticoagulant toxic to cells; Insufficient number of cells; Improper fixative

Reference Range

In a normal cell the expected pattern is a two aqua, two orange and two green signal pattern. An abnormal cell containing the t(8;14) shows a pattern of one orange, one green, two orange/green fusions, and two aqua signals.

Description

The t(8;14)(q32;q24) joins the cellular oncogenes C-MYC with sequences from immunoglobulin heavy chain gene enhancers leading to deregulated expression of C-MYC protein. This abnormality is characteristic of Burkitt lymphoma and may be seen with other chromosomal abnormalities in cases of large B-cell lymphoma. FISH can detect this translocation in either interphase or metaphase cells.

References
  1. Janz S. DNA Repair (Amst). 2006 Sep 8;5(9-10):1213-24.
  2. Pienkowska-Grela et al (2005). Cancer Genet Cytogenet 156(2):114.