F IGH MYC*; FP IGH MYC*
t(8;14)(q32;q24), MYC
Burkitt lymphoma
Fluorescence in situ Hybridization (FISH)
*Performed by affiliate laboratory
7-9 days
F IGH MYC
5mL peripheral blood in sodium heparin
3mL bone marrow in sodium heparin
Fixed cytogenetically prepared cells in sterile centrifuge tube with pellet visible in 3:1, Methanol:Acetic Acid
FP IGH MYC
FFPE tissue is acceptable for FISH analysis. Preferred fixative is 10% neutral buffered formalin. Tissues preserved in B5 fixative or decalcified are usually not suitable for FISH. Tumor sections cut 3-5 µm thick and mounted on positively charged organosilane coated (silanized) slides work well. Request several unstained sections (two for each probe) and one H&E stained slide
4°C to 25°C during transit, but specimens may be transported on refrigerated gel packs. Do not allow the gel pack to come in contact with the specimen. Do not freeze. Extreme temperatures should be avoided.
Clotted specimen; Specimen exposed to extreme temperature; Anticoagulant toxic to cells; Insufficient number of cells; Improper fixative
In a normal cell the expected pattern is a two aqua, two orange and two green signal pattern. An abnormal cell containing the t(8;14) shows a pattern of one orange, one green, two orange/green fusions, and two aqua signals.
The t(8;14)(q32;q24) joins the cellular oncogenes C-MYC with sequences from immunoglobulin heavy chain gene enhancers leading to deregulated expression of C-MYC protein. This abnormality is characteristic of Burkitt lymphoma and may be seen with other chromosomal abnormalities in cases of large B-cell lymphoma. FISH can detect this translocation in either interphase or metaphase cells.