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Melanocytic Markers by Immunohistochemistry (IHC)

Test Code

See associations below

Associations
Test Code Description
 I CK5/P63 Cytokeratin 5/P63, tumor suppressor gene cocktail
I CK8/18 cytokeratin 8/18, adenocarcinoma
I HMB45 Melanoma associated marker
I MC Melanoma cocktail (HMB-45, MelanA)
I MELANA Melanoma associated marker/Mart 1
I P16 p16 tumor suppresor protein
I P63NU p63 tumor suppressor gene protein nuclear
I SMM Smooth muscle myosin
I S100 S100 protein
I TYR Tyrosinase
I VIM vimentin
  * Performed at affiliate laboratory
I CLLG IV Collagen IV, basement membrane protein*

 

Methodology

Immunohistochemistry
Technical component (TC) available for all markers

Turnaround Time

2 days

Specimen Requirements
  • Formalin fixed tissue
  • Formalin fixed paraffin embedded tissue (preferred)
Specimen Stability
Indefinitely at room temperature
Storage & Handling
  • Ship ambient.
  • In extreme hot weather, ship with a cool pack.
Causes for Rejection

Inadequate fixation; Improper labeling

Reference Range

Marker and tissue specific

Description

Results are reviewed by a board-certified pathologist to determine if tissues are positive or negative for the marker. These results may be used to assist in determining appropriate patient therapy or treatment.
Immunohistochemistry combines the principles of histochemistry with the high degree of molecular specificity of the antibody-antigen reaction. The use of an enhanced-polymer detection system provides many benefits including increased sensitivity, fewer procedural steps as compared to conventional techniques, and a reduction in non-specific background staining.
 
MPLN offers a range of markers by Immunohistochemistry including hematologic, tissue specific, infectious agents and proliferation antigens.

References
  1. Press M et al. (2002). Steroids. 67:799-813.
  2. Perttschuk et al. (1996). Cancer. 77:2514-15.
  3. Battifora et al. (1993). Appl Immunohistochem. 1:39-45.