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MYB 6q deletion by FISH

Test Code

F MYB

Test Synonyms

deletion 6q23, del(6)(q23)

Associations

Chronic lymphocytic leukemia (CLL), Childhood acute lymphoblastic leukemia (ALL), B-cell small lymphocytic lymphoma (SLL), Plasma cell myeloma

Methodology

Fluorescence in situ Hybridization (FISH)

Turnaround Time

3-5 days

Specimen Requirements

2.0 mL (min. 1.0 mL) peripheral blood in sodium heparin preferred, EDTA accepted
1.0 mL (min. 0.5 mL) bone marrow in sodium heparin preferred, EDTA accepted
5 mm^3 fresh tissue or 3.0 mL (min. 2.0 mL) FNA in MPLN RPMI media

Specimen Stability
Peripheral blood and bone marrow stable at 18-25°C for 72 hours
Fresh tissue or FNA stable at 2-8°C for 72 hours
Storage & Handling

Whole blood and bone marrow, ship ambient
Fresh tissue or FNA ship in a Styrofoam container with a cool/refrigerated pack (Do not allow cool pack to directly contact sample)

Causes for Rejection

Clotted specimen; Specimen exposed to extreme temperature; Anticoagulant toxic to cells; Insufficient number of cells

Reference Range

A normal cell will contain a two aqua signal pattern. In individuals with a deletion of the 6q23, there is a single aqua signal.

Description

This abnormality has been associated with childhood acute lymphoblastic leukemia (ALL), adult ALL, B-cell small lymphocytic lymphoma, atypical chronic lymphocytic leukemia and plasma cell myeloma. FISH can detect this rearrangement in either interphase or metaphase cells.

References
  1. Letter to the editor. (2003). Cancer Genetics and Cytogenetics. 143 87–88.
  2. Brigaudeau C and Bilhou-Nabera C.(1998). del(6q) abnormalities in lymphoid malignancies. Atlas Genet Cytogenet Oncol Haematol. December.
  3. Brigaudeau C. (1988) del(6q) in Multiple Myeloma. Atlas Genet Cytogenet Oncol Haematol. September.