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Trisomy 5, 9,15 by FISH

Test Code

F T5T9T15

Test Synonyms

+5p/+9/+15

Associations

Multiple Myeloma

Methodology

Fluorescence in situ Hybridization (FISH)

Turnaround Time

3-5 days

Specimen Requirements

2.0 mL (min. 1.0 mL) peripheral blood in sodium heparin preferred, EDTA accepted
1.0 mL (min. 0.5 mL) bone marrow in sodium heparin preferred, EDTA accepted
5 mm^3 fresh tissue or 3.0 mL (min. 2.0 mL) FNA in MPLN RPMI media

Specimen Stability
Peripheral blood and bone marrow stable at 18-25°C for 72 hours
Fresh tissue or FNA stable at 2-8°C for 72 hours
Storage & Handling

Whole blood and bone marrow, ship ambient
Fresh tissue or FNA ship in a Styrofoam container with a cool/refrigerated pack (Do not allow cool pack to directly contact sample)

Causes for Rejection

Clotted specimen; Specimen exposed to extreme temperature; Anticoagulant toxic to cells; Insufficient number of cells

Reference Range

In a normal cell a two green, two aqua and two orange signal pattern is observed. In an abnormal cell containing hyperdiploidy of either chromosome 5, chromosome 9 or chromosome 15, greater than two signals is observed for the respective chromosomes.

Description

Aneuploidy is a frequent finding in plasma cell myeloma.  Approximately 60-70% of myeloma cases have been reported to have hyperdiploid clones. About 10-20% have been reported to have pseudodiploid clones, and 10-30% have been reported to have hypodiploid clones. FISH can detect these abnormalities and interphase and metaphase cells.

References
  1. Fonseca et al. (2004). Genetics and cytogenetics of multiple myeloma: A workshop report. Cancer Res. 64:1546. Hideshima et al. (2004). Advances in biology of multiple myeloma: clinical applications. Blood. 104:607.
  2. Pratt G. (2002). Molecular aspects of multiple myeloma. J Clin Pathol: Mol Pathol. 55:273.
  3. Schmidt-Wolf et al. (2006). Cancer Genetics and Cytogenetics. 167:20–25.