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Bladder Cancer Panel +3,+7,+17,9p21- by FISH

Test Code

F URO

Test Synonyms

+3,+7,+17, del(9)(p21)

Associations

Bladder Cancer

Methodology

Fluorescence in situ Hybridization (FISH)

Turnaround Time

3-5 days

Specimen Requirements

50 mL (min. 35 mL) of voided urine in PreservCyt®. Add PreservCyt at a 2:1 ratio of urine to PreservCyt.

MPLN recommends collecting a second-morning voided urine sample.  Urothelial cells may degenerate while in the urine overnight, which may interfere with FISH hybridization and interpretation

Specimen Stability
Specimen stable at 4-30°C for 48 hrs.
Storage & Handling

Store urine or bladder washing at 4-30°C immediately after collection. Ship overnight in Styrofoam container with a cool/refrigerated pack. (Do not allow the sample to be in direct contact with the cool/refrigerated pack.)

Causes for Rejection

Any non-urine specimen including bladder or renal pelvic washings; Incorrect preservative; Sample exposed to extreme temperature; Insufficient volume or number of cells for analysis; Specimen transport greater than 48 hours

Reference Range

In a normal cell two orange, two green, two aqua and two gold signals will be seen. A case is considered abnormal when at least 2 of 3 signals (orange, green, or aqua) is seen in greater than or equal to 4 cells or if there is an absence of both gold signals in greater than or equal to 12 cells.

Description

+3,+7,+17, del(9)(p21) is recommended for the evaluation of patients with hematuria who are suspected of having bladder cancer and for monitoring patients for tumor recurrence. An important advantage in the evaluation of bladder cancer patients is that the chromosome changes detected by FISH may predate the cytological changes by several months.

References
  1. Phillips and Richardson. (2006). Aneuploidy in bladder cancers: the utility of fluorescent in situ hybridization in clinical practice. BJU Inter.98:33-37.
  2. Sarosdy et al. (2006). Use of a multi-target fluorescence in situ hybridization assay to diagnose bladder cancer in patients with hematuria. 176:44-47.
  3. Skacel et al. (2003). Multitarget fluorescence in situ hybridization assay detects transitional cell carcinoma in the majority of patients with bladder cancer and atypical or negative urine cytology. J Urol. 169:2101.
Trademarks

PreservCyt is a trademark of Hologic, Inc.