Chronic lymphocytic leukemia(CLL); IgVH mutational status
Flow cytometry, multiparameter analysis
5.0 mL (min. 2.0 mL) heparin blood preferred, EDTA and ACD whole blood accepted
3.0 mL (min. 1.0 mL) heparin bone marrow aspirate preferred, EDTA bone marrow accepted
Whole blood or bone marrow, ship ship in a Styrofoam container with a cool pack. (Do not allow cool/refrigerated pack to directly contact samples)
Specimen >48 hours old prior to testing; Specimens stored at incorrect temperature; Non-viable specimens; Specimens in inappropriate anticoagulant; Too few cells; Hemolysis; Specimen clotted
ZAP-70 can be measured by flow cytometry and has been reported to correlate with IgVH mutational status and outcomes in cases of classic chronic lymphocytic leukemia patients (CLL). Higher levels of ZAP-70 in neoplastic CLL cells have been reported to be associated with a less favorable clinical course. ZAP-70 evaluation, in conjunction with FISH analysis and clinical parameters, may help identify subsets of patients with an increased risk of progression. ZAP-70 testing is performed on peripheral blood and bone marrow samples.