F AML ETO
AML1/ETO t(8;21)(q22;q22); RUNX1T1
Acute myelogenous leukemia, AML(M2)
Fluorescence in situ Hybridization (FISH)
5mL peripheral blood in sodium heparin
3mL bone marrow in sodium heparin
Fixed cytogenetically prepared cells in sterile centrifuge tube with pellet visible in 3:1, Methanol:Acetic Acid
4°C to 25°C during transit, but specimens may be transported on refrigerated gel packs. Do not allow the gel pack to come in contact with the specimen. Do not freeze. Extreme temperatures should be avoided.
Clotted specimen; Specimen exposed to extreme temperature; Anticoagulant toxic to cells; Insufficient number of cells
FISH results indicate whether rearrangement is present or absent.
In a normal cell, two orange and two green signals will be detected. In a cell with the t(8;21), two fusion signals will be seen representing the two rearranged chromosomes along with one orange and one green signal.
The t(8;21)(q22;q22) is found in 12% of all acute myeloid leukemia (AML) cases, including 40–50% of AML M2 subtype and a small portion of M0, M1 and M4 subtypes of the FAB classification.
FISH can detect this translocation in either interphase or metaphase cells.