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C MYC 8q24 Rearrangement by FISH

Test Code

F CMYC, FP CMYC

Test Synonyms

MYC, 8q24 translocation

Associations

Lymphoma, Burkitt, Diffuse large B-cell, Solid tumors, Breast and prostate cancer

Methodology

Fluorescence in situ Hybridization (FISH)

Turnaround Time

3 -5 days

Specimen Requirements

F CMYC
5mL peripheral blood in sodium heparin
3mL bone marrow in sodium heparin
Fixed cytogenetically prepared cells in sterile centrifuge tube with pellet visible in 3:1, Methanol:Acetic Acid

FP CMYC
FFPE tissue is acceptable for FISH analysis. Preferred fixative is 10% neutral buffered formalin. Tissues preserved in B5 fixative or decalcified are usually not suitable for FISH. Tumor sections cut 3-5 µm thick and mounted on positively charged organosilane coated (silanized) slides work well. Request several unstained sections (two for each probe) and one H&E stained slide

Specimen Stability
Blood and bone marrow = 4°C to 25°C, specimens are stable up to 72 hours
Fixed cell pellets are stable for years when stored at -28°C to 15°C
FFPE Stable indefinitely when stored at 20°C to 25°C
Storage & Handling

4°C to 25°C during transit, but specimens may be transported on refrigerated gel packs. Do not allow the gel pack to come in contact with the specimen. Do not freeze. Extreme temperatures should be avoided.

Causes for Rejection

Clotted specimen; Specimen exposed to extreme temperature; Anticoagulant toxic to cells; Insufficient number of cells;
Improper fixative

Reference Range

A normal nucleus hybridized with the MYC rearrangement probe set produces a two orange/green (yellow) fusion pattern.

The 8q24 rearrangement in t(2;8), t(8;22) or t(8;14) will generate one orange, one green, and one fusion pattern.

Description

Translocations of chromosome 8 are common aberrations in the B-cell non-Hodgkin lymphoma group. The presence of the typical t(8;14) or the rare variants t(8;22) and t(2;8) has been confirmed in all cases of Burkitt lymphoma and in some cases of Burkitt-like lymphoma and diffuse large B-cell lymphoma. FISH can detect this rearrangement in either interphase or metaphase cells.

References
  1. Pienkowska-Grela et al. (2005). Cancer Genet Cytogenet. 156(2):114.