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Epstein Barr Virus by ISH

Test Code


Test Synonyms



Infectious mononucleosis, Burkitt's lymphoma,Reed Sternberg cells Hodgkin's lymphoma, nasopharyngeal carcinoma, HIV infection


Chromogenic in-situ Hybridization (CISH)

Turnaround Time

2 days

Specimen Requirements

Paraffin embedded tissue block
3 slides (3-5 uM) per marker on adhesion glass

Specimen Stability
Indefinitely at room temperature
Storage & Handling

Ship ambient. Protect from extreme temperature with an ice pack. Separate ice pack from specimen.

Causes for Rejection

Improper specimen labeling; Inadequate tissue evaluation; Improper sample submission

Reference Range

EBER by ISH is performed on formalin-fixed paraffin-embedded tissues and the result is very similar to the traditional IHC slide -  a brown (DAB) chromogen target location on a Hematoxylin counter-stained slide.


EBV infection is associated with a variety of pathological conditions. The virus has been reported to be demonstrated in infectious mononucleosis, Burkitt’s lymphoma, the Reed Sternberg cells of Hodgkin’s disease and in nasopharyngeal carcinoma. In HIV infection, EBV has also been reported to be demonstrated in primary CNS lymphomas and oral hairy leukoplakia lesions. 

The technique demonstrates cells latently-infected with EBV as it hybridizes to abundantly expressed Epstein-Barr virus-encoded RNA (EBER) transcripts which are concentrated in the nuclei of latently-infected cells.


McNicol AM, Farquharson MA. (1997)In situ hybridization and its diagnostic applications in pathology. J Pathol.182(3):250-61.