» Test Menu » IGH/MYC t(8;14) by FISH* Client Login | Email | Phone: 1.865.380.9746

IGH/MYC t(8;14) by FISH*

Test Code


Test Synonyms

t(8;14)(q32;q24), MYC


Burkitt lymphoma



Fluorescence in situ Hybridization (FISH)
*Performed by affiliate laboratory

Turnaround Time

7-9 days

Specimen Requirements

5mL peripheral blood in sodium heparin
3mL bone marrow in sodium heparin
Fixed cytogenetically prepared cells in sterile centrifuge tube with pellet visible in 3:1, Methanol:Acetic Acid

FFPE tissue is acceptable for FISH analysis. Preferred fixative is 10% neutral buffered formalin. Tissues preserved in B5 fixative or decalcified are usually not suitable for FISH. Tumor sections cut 3-5 µm thick and mounted on positively charged organosilane coated (silanized) slides work well. Request several unstained sections (two for each probe) and one H&E stained slide

Specimen Stability
Blood and bone marrow = 4°C to 25°C, specimens are stable up to 72 hours
Fixed cell pellets are stable for years when stored at -28°C to 15°C
FFPE Stable indefinitely when stored at 20°C to 25°C
Storage & Handling

4°C to 25°C during transit, but specimens may be transported on refrigerated gel packs. Do not allow the gel pack to come in contact with the specimen. Do not freeze. Extreme temperatures should be avoided.

Causes for Rejection

Clotted specimen; Specimen exposed to extreme temperature; Anticoagulant toxic to cells; Insufficient number of cells; Improper fixative

Reference Range

In a normal cell the expected pattern is a two aqua, two orange and two green signal pattern. An abnormal cell containing the t(8;14) shows a pattern of one orange, one green, two orange/green fusions, and two aqua signals.


The t(8;14)(q32;q24) joins the cellular oncogenes C-MYC with sequences from immunoglobulin heavy chain gene enhancers leading to deregulated expression of C-MYC protein. This abnormality is characteristic of Burkitt lymphoma and may be seen with other chromosomal abnormalities in cases of large B-cell lymphoma. FISH can detect this translocation in either interphase or metaphase cells.

  1. Janz S. DNA Repair (Amst). 2006 Sep 8;5(9-10):1213-24.
  2. Pienkowska-Grela et al (2005). Cancer Genet Cytogenet 156(2):114.