Test Code

F MYB

Test Synonyms

deletion 6q23, del(6)(q23)

Associations

Chronic lymphocytic leukemia (CLL), Childhood acute lymphoblastic leukemia (ALL), B-cell small lymphocytic lymphoma (SLL), Plasma cell myeloma

Methodology

Fluorescence in situ Hybridization (FISH)

Turnaround Time

3-5 days

Specimen Requirements

5mL peripheral blood in sodium heparin
3mL bone marrow in sodium heparin
Fixed cytogenetically prepared cells in sterile centrifuge tube with pellet visible in 3:1, Methanol:Acetic Acid

Specimen Stability

Storage & Handling

4°C to 25°C during transit, but specimens may be transported on refrigerated gel packs. Do not allow the gel pack to come in contact with the specimen. Do not freeze. Extreme temperatures should be avoided.

Causes for Rejection

Clotted specimen; Specimen exposed to extreme temperature; Anticoagulant toxic to cells; Insufficient number of cells

Reference Range

A normal cell will contain a two aqua signal pattern. In individuals with a deletion of the 6q23, there is a single aqua signal.

Description

This abnormality has been associated with childhood acute lymphoblastic leukemia (ALL), adult ALL, B-cell small lymphocytic lymphoma, atypical chronic lymphocytic leukemia and plasma cell myeloma. FISH can detect this rearrangement in either interphase or metaphase cells.

References

1. Letter to the editor. (2003). Cancer Genetics and Cytogenetics. 143 87–88.
2. Brigaudeau C and Bilhou-Nabera C.(1998). del(6q) abnormalities in lymphoid malignancies. Atlas Genet Cytogenet Oncol Haematol. December.
3. Brigaudeau C. (1988) del(6q) in Multiple Myeloma. Atlas Genet Cytogenet Oncol Haematol. September.