1p-, 1q+, 5,+9, t(11;14), 13q-, +15, 17p-, reflex t(4;14) / t(14;16)
Fluorescence in situ Hybridization (FISH)
1p32.3 (CDKN2C)/1q21 (CKS1B)
5p15.31 (D5S630,D5S2064)/5q31.2 (EGR1)
CEP 9 (D9Z1)
11q13 (CCND1)/14q32.3 (IGH)
CEP 15 (D15Z4)
17p13 (TP53)/CEP17 (D17Z1)
5mL peripheral blood in sodium heparin
3mL bone marrow in sodium heparin
Fixed cytogenetically prepared cells in sterile centrifuge tube with pellet visible in 3:1, Methanol:Acetic Acid
4°C to 25°C during transit, but specimens may be transported on refrigerated gel packs. Do not allow the gel pack to come in contact with the specimen. Do not freeze. Extreme temperatures should be avoided.
Clotted specimen; Specimens exposed to extreme temperature; Anticoagulant toxic to cells; Insufficient number of cells
Cytogenetic studies using chromosome analysis and interphase fluorescence in situ hybridization (FISH) are routine procedures for prognostic stratification of plasma cell myeloma. Several cytogenetic abnormalities such as t(4;14), del(17/17p), t(14;16), nonhyperdiploidy, and gain(1q) were shown to confer poor prognosis. Bone marrow samples used for plasma cell neoplasia testing are sorted for CD138 positive selection cells using magnetic beads. FISH analysis on the CD138 positive selected cells has been shown to increase diagnostic sensitivity.
In cases with inadequate /insufficient sample, CD138+ enrichment will not be attempted.”