Test Code

I P16

Test Synonyms

Tumor suppressor gene, Cyclin-dependent kinase inhibitor 2A, CDKN2A, p16Ink4A

Associations

Head and Neck squamous cancer, myeloma, bladder cancer, cervical cancer. gastric cancer

Methodology

Immunohistochemistry

Turnaround Time

2 days

Specimen Requirements

Paraffin embedded tissue block
3 slides (3-5 uM) per marker on adhesion glass

Specimen Stability

Storage & Handling

Ship ambient. Protect from extreme temperature with an ice pack. Separate ice pack from specimen.

Causes for Rejection

Inadequate fixation; Improper labeling

Description

P16 plays an important role in regulating the cell cycle, and mutations in p16 increase the risk of developing a variety of cancers.

References

1. Romagosa C et al.(2011.p16Ink4a overexpression in cancer: a tumor suppressor gene associated with senescence and high-grade tumors.Oncogene 30:2087-2097
2. Kian Ang K et al. (2010).Human Papillomavirus and Survival of Patients with Oropharyngeal Cancer. N Engl J Med 363:24-35.
3. Lewis J,S et al. (2010). p16 Positive Oropharyngeal Squamous Cell Carcinoma: An Entity With a Favorable Prognosis Regardless of Tumor HPV Status. Am J Surg Pathol 34:1088–1096