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Prognostic Markers by Immunohistochemistry (IHC)

Test Code

See associations below

Associations
Test Code Description
I HER2 HER2/neu (IVD)
I HER2 GA HER2/neu Gastric cancer
I MSI Microsatellite instability - MLH1, MSH2, MSH6
I P53 p53 tumor suppressor gene protein
  *performed at affiliate laboratory
I CATH D* Cathepsin D, breast carcinoma

 

Methodology

Immunohistochemistry
Technical component (TC) available for all markers

Turnaround Time

2 days

Specimen Requirements

Paraffin embedded tissue block
3 slides (3-5 uM) per marker on adhesion glass


Please note:
If submitting HER2 protein over-expression by immunohistochemistry,
HER2(ERBB2) gene amplification by in situ hybridization or estrogen /
progesterone receptor expression by immunohistochemistry, the specimen must
follow fixation guidelines listed below:
1. Specimens should be immersed in fixative within one hour of the biopsy or
resection.
2. If delivery of a resection specimen to the pathology department is delayed (eg,
specimens from remote sites), the tumor should be bisected prior to the
immersion in fixative. In such cases, it is important that the surgeon ensure
that the identity of the resection margins is retained in the bisected specimen;
alternatively, the margins may be separately submitted.
3. The time of removal of the tissue and the time of immersion of the tissue in
fixative should be recorded and submitted to the laboratory.

Specimen Stability
Indefinitely at room temperature
Storage & Handling

Ship ambient. Protect from extreme temperature with ice pack. Separate ice pack from specimen.

Causes for Rejection

Marker and tissue specific

Description

Results are reviewed by a board-certified pathologist to determine if tissues are positive or negative for the marker. These results may be used to assist in determining appropriate patient therapy or treatment.
Immunohistochemistry combines the principles of histochemistry with the high degree of molecular specificity of the antibody-antigen reaction. The use of an enhanced-polymer detection system provides many benefits including increased sensitivity, fewer procedural steps as compared to conventional techniques, and a reduction in non-specific background staining. 


MPLN offers a range of markers by Immunohistochemistry including hematologic, tissue specific, infectious agents and proliferation antigens.

References
  1. Press M et al. (2002). Steroids. 67:799-813.
  2. Perttschuk et al. (1996). Cancer. 77:2514-15.
  3. Battifora et al. (1993). Appl Immunohistochem. 1:39-45.