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Trisomy 8 by FISH

Test Code

F T8

Test Synonyms

+8, CEP 8

Associations

Acute myeloid leukemia (AML), Chronic myelogenous leukemia (CML), Myelodysplastic syndrome (MDS)

Methodology

Fluorescence in situ Hybridization (FISH)

Turnaround Time

3-5 days

Specimen Requirements

5mL peripheral blood in sodium heparin
3mL bone marrow in sodium heparin
Fixed cytogenetically prepared cells in sterile centrifuge tube with pellet visible in 3:1, Methanol:Acetic Acid

Specimen Stability
Blood and bone marrow = 4°C to 25°C, specimens are stable up to 72 hours
Fixed cell pellets are stable for years when stored at -28°C to 15°C
Storage & Handling

4°C to 25°C during transit, but specimens may be transported on refrigerated gel packs. Do not allow the gel pack to come in contact with the specimen. Do not freeze. Extreme temperatures should be avoided.

Causes for Rejection

Clotted specimen; Specimen exposed to extreme temperature; Anticoagulant toxic to cells; Insufficient number of cells

Reference Range

Normal cells have two aqua signals. Abnormal cells containing trisomy 8, have a three aqua signal pattern.

Description

Trisomy 8 is relatively specific for myeloid disorders and is rarely observed in lymphoid disease. It is found in 10-15% of patients with acute myeloid leukemia (AML), 15-20% of patients with myelodysplastic syndromes (MDS), as a secondary abnormality in Philadelphia chromosome positive CML, and in other myeloproliferative disorders. Trisomy 8 occurs as the sole chromosome abnormality in about 40% of AML cases, occurs in combination with simple chromosome changes in 35% of cases, and as part of a complex karyotype in 25% of AML patients.

References
  1. Matsuoka H et al. (2005). Establishment of a human myeloid cell line with trisomy 8 derived from overt leukemia following myelodysplastic syndrome.
  2. Wolman SR. (2002). Impact of trisomy 8 (+8) on clinical presentation, treatment response, and survival in acute myeloid leukemia: a Southwest Oncology Group study. Blood. 100(1):29.
  3. Huret JL.(November 1998) +8 or trisomy 8. Atlas Genet Cytogenet Oncol Haematol.