+8, CEP 8
Acute myeloid leukemia (AML), Chronic myelogenous leukemia (CML), Myelodysplastic syndrome (MDS)
Fluorescence in situ Hybridization (FISH)
5mL peripheral blood in sodium heparin
3mL bone marrow in sodium heparin
Fixed cytogenetically prepared cells in sterile centrifuge tube with pellet visible in 3:1, Methanol:Acetic Acid
4°C to 25°C during transit, but specimens may be transported on refrigerated gel packs. Do not allow the gel pack to come in contact with the specimen. Do not freeze. Extreme temperatures should be avoided.
Clotted specimen; Specimen exposed to extreme temperature; Anticoagulant toxic to cells; Insufficient number of cells
Normal cells have two aqua signals. Abnormal cells containing trisomy 8, have a three aqua signal pattern.
Trisomy 8 is relatively specific for myeloid disorders and is rarely observed in lymphoid disease. It is found in 10-15% of patients with acute myeloid leukemia (AML), 15-20% of patients with myelodysplastic syndromes (MDS), as a secondary abnormality in Philadelphia chromosome positive CML, and in other myeloproliferative disorders. Trisomy 8 occurs as the sole chromosome abnormality in about 40% of AML cases, occurs in combination with simple chromosome changes in 35% of cases, and as part of a complex karyotype in 25% of AML patients.