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B-cell IGH Gene Rearrangement by NGS

Test Code


Test Synonyms

B-cell gene rearrangement


B-cell leukemia, Lymphoma


Next Generation Sequencing (NGS)

Turnaround Time

5-7 days

Specimen Requirements

5.0 mL (min. 3.0 mL) whole blood EDTA or Sodium Heparin
3.0 mL (min. 1.0 mL) bone marrow EDTA or Sodium Heparin
5 mm^3 bone marrow core biopsy, fresh tissue or frozen tissue in MPLN RPMI
3.0 mL (min. 2.0 mL) FNA in MPLN RPMI medium
Formalin fixed paraffin embedded tissue

Specimen Stability
EDTA whole blood or bone marrow stable at 18-25°C for 72 hours
Bone marrow biopsy, fresh tissue or FNA in MPLN RPMI at 2-8°C stable for 72 hours
Frozen tissue frozen at 20°C stable indefinitely
Paraffin embedded tissue at 18-25°C stable indefinitely
Storage & Handling

EDTA whole blood, bone marrow, bone marrow biopsy, fresh tissue or FNA, ship in a Styrofoam container with a cool/refrigerated pack. (Do not allow the cool pack to directly contact the sample.)
Frozen tissue, ship on dry ice.
Paraffin embedded tissue, ship ambient.

Causes for Rejection

Improper specimen labeling; Insufficient sample volume; Clotted specimen; Specimen older than 7 days

Reference Range

Immunoglobulin Heavy Chain Gene (IGH) V-J Regions


Evaluation for suspected clonal B-cell lymphoproliferations when morphologic, immunochemical and/or flow cytometry analyses are inconclusive.

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