1p36-/19q13-, del(1)(p36), del(19)(q13)
Fluorescence in situ Hybridization (FISH)
10% neutral buffered formalin fixed paraffin embedded tissue
5 mm^3 fresh tissue or 3.0 mL (min. 2.0 mL) FNA in MPLN RPMI media
Paraffin blocks stable at 18-25°C indefinitely
Fresh tissue or FNA stable at 2-8°C for 72 hours
Storage & Handling
Fresh tissue, FNA or paraffin embedded tissue, ship in a Styrofoam container with a cool/refrigerated pack (Do not allow cool pack to directly contact sample)
Causes for Rejection
Improper fixative; Tissue not fixed; Insufficient tissue; Specimen exposed to extreme temperature
Deletions of chromosome 1p and 19q arms are frequent genetic abnormalities in primary human gliomas and are especially common in oligodendrogliomas. While 1p deletions are relatively common in other types of tumors, 19q deletions appear to be common only in gliomas.
- Law, Mark et al. (2006). Cancer Genetics and Cytogenetics. 160:1–14.
- Barbashina V et al. (2005). Allelic loss at 1p36 and 19q13 in gliomas: correlation with histologic classification, definition of a 150-kb minimal deleted region at 1p36, and evaluation of CAMTA1 as a candidate tumor suppressor gene. Clin Cancer Research. 11:1119.
- Smith JS et al. (2000). Alterations in chromosome arms 1p and 19q as predictors of survival in oligodendrogliomas, astrocytomas, and mixed oligoastrocytomas. J Clin Oncol. 18(3):636.