CD28 family of receptors, CTLA-4 cytotoxic T Lymphocyte-associated antigen 4, ICOS inducible costimulator, and B- and T-lymphocyte attenuator.
Immunohistochemistry, Technical component (TC) available
This assay utilizes the NAT105 mouse monoclonal antibody for immunohistochenical detection of the immune regulatory molecule programmed death-1 (PD-1).
Formalin fixed paraffin embedded tissue block
Bone Marrow core in formalin
Biopsy in formalin
Unstained Previously Prepared Slides (paraffin sections on slides)
Transport at ambient temperature.
In extreme hot weather, ship with a cool pack
Improper specimen labeling; Insufficient sample; Inadequate fixation and/or processing
Quantitative in Tumor Infiltrating Lymphocytes (TILs): Cut off POSITIVE in =/>1% of tumor infiltrating lymphocytes (TILs); NEGATIVE (no reactivity in tumor infiltrating lymphocytes, <1%)
Qualitative PD-1 immunoreactivity is demonstrated in x% of lymphocytes
Detection of PD-1 protein expression by immunohistochemistry has utility in both hematopathology diagnostics and in cancer biology. The programmed death-1 (PD-1) protein is expressed by follicular helper T-cells, and is useful in the diagnosis of hematolymphoid malignancies such as angioimmunoblastic T-cell lymphoma (AITL). In addition, clinical evidence suggests that IHC staining of the PD-1/PD-L1 pathway may have prognostic and/or therapeutic significance in some cancer patients.