T-cell receptor gamma (TCRγ) gene rearrangement, T cell gene rearrangement
Cutaneous Lymphoproliferative Disorders
Molecular characterization of T-cell receptor gene rearrangements and immunoglobulin heavy and light chain gene rearrangement detection is accomplished with reliable and robust tests to identify clonal T-cell and B-cell populations in a variety of cutaneous lymphoproliferative disorders.<br><br>
While the demonstration of a clonal T/B cell population is not necessarily reflective of a truly pathologic process, positive results interpreted in the context of all laboratory findings can support diagnosis of malignancy. The ability to routinely perform these studies make these highly sensitive and specific tests well suited for the evaluation of these conditions.
Polymerase chain reaction (PCR), capillary gel electrophoresis
5.0 mL (min. 3.0 mL) whole blood EDTA or Sodium Heparin
3.0 mL (min. 1.0 mL) bone marrow EDTA or Sodium Heparin
5 mm^3 bone marrow core biopsy, fresh tissue or frozen tissue in MPLN RPMI
3.0 mL (min. 2.0 mL) FNA in MPLN RPMI medium
Formalin fixed paraffin embedded tissue
Whole blood or bone marrow stable at 18-25°C for 72 hours
Bone marrow biopsy, fresh tissue or FNA in MPLN RPMI stable at 2-8°C for 72 hours
Frozen tissue stable at -20°C indefinitely
Paraffin embedded tissue stable at 18-25°C indefinitely
Storage & Handling
Whole blood, bone marrow, bone marrow biopsy, fresh tissue or FNA, ship in a Styrofoam container with a cool/refrigerated pack (Do not allow the cool pack to directly contact the sample.)
Frozen tissue, ship on dry ice
Paraffin embedded tissue, ship ambient
Causes for Rejection
Insufficient volume; Specimen at incorrect temperature, Incorrect anticoagulant or fixative
No rearrangement detected (germline)
Evaluation for suspected clonal T-cell lymphoproliferations when morphologic, immunochemical, and/or flow cytometry analyses are inconclusive.
- Bruggemann M et al. (2007). Powerful strategy for polymerase chain reaction-based clonality assessment in T-cell malignancies. Report of the BIOMED-2 Concerted Action CT98-3936. Leukemia. 21:215-221.
- Langerak AW et al. (2007). Polymerase chain reaction-based clonality testing in tissue samples with reactive lymphoproliferations: usefulness and pitfalls. Report of the BIOMED-2 Concerted Action BMH4-CT98-3936. Leukemia. 21:222-229.
- Liu H et al. (2007). A practical strategy for the routine use of BIOMED-2 PCR assays for the detection of B- and T-cell clonality in diagnostic haematopathology. Br J Haematol. 138:31-43.
- Van Krieken JHJM et al. (2007). Improved reliability of lymphoma diagnosis via PCR-based clonality testing: Report of the BIOMED-2 Concerted Action BHMA-CT98-3936. Leukemia. 21:201-206.
- Saberg Y et al. (2003). Molecular immunoglobulin/T-cell receptor clonality analysis in cutaneous lymphoproliferations. Experience with the BIOMED-2 standardized polymerase chain reaction protocol. Haematologica. 88:659-670.
- Van Dongen JJM et al. (2003). Design and standardization of PCR primers and protocols for detection and clonal immunoglobulin and T cell receptor gene recombinations in suspect lymphoproliferations: Report of the BIOMED-2 Concerted Action BMH4-CT98-3936. Leukemia. 17:2257-2317.