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T-cell Subsets (CD3, CD4, CD8, CD4:CD8 ratio) by FLOW*

Test Code


Test Synonyms

CD4/CD8 count; T helper / T suppressor analysis; lymphocyte subsets




Flow cytometry, multiparameter analysis

* Performed by affiliate laboratory

Turnaround Time

3 days

Specimen Requirements

4.0 mL (min. 0.5mL) whole blood EDTA (Lavender), K EDTA (pink), or sodium or lithium heparin (green)

Specimen Stability
Specimen stable at 18-25°C for 72 hours (EDTA) and 48 hours (Heparin)
Storage & Handling

Ship ambient. (During the summer, a cool/refrigerated pack may be used if it is not allowed to contact the sample.)

Causes for Rejection

Specimens stored at incorrect temperature; Non-viable specimens; Specimens in inappropriate anticoagulant; Too few cells; Hemolysis; Specimen clotted;
BAL samples are not appropriate for this assay

Reference Range

See report

  1. Maurice R. G. O’Gorman and Janet K. A. Nicholson. (2000). Adoption of single-platform technologies for enumeration of absolute T-lymphocyte subsets in peripheral blood. Clin Diagn Lab Immunol. 7(3):333–335.